![Western Blot: DAT1 [p Thr53] Antibody [NBP2-29533] - Western blot of rat caudate lysate showing specific immunolabeling of the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).](http://images.novusbio.com/fullsize/DAT1-[p-Thr53]-Antibody-Western-Blot-NBP2-29533-img0001.jpg "Western Blot: DAT1 [p Thr53] Antibody [NBP2-29533] - Western blot of rat caudate lysate showing specific immunolabeling of the ~55k glycosylated form of the DAT protein phosphorylated at Thr53. Immunolabeling is blocked by the phospho-peptide used as antigen (peptide), but not by the corresponding dephospho-peptide (not shown).")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr53 conjugated to KLH. Accession # P23977 |
| Modification | p Thr53 |
| Specificity | Specific for endogenous levels of the ~55 kDa glycosylated form of the DAT protein phosphorylated at Thr53. Relative mobility may vary depending on the state of glycosylation of the DAT protein. The antibody works best in lysates that have not been boiled prior to being run on an SDS-PAGE gel. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide. |
| Preparation Method |
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC6A3 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Preservative | No Preservative |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for SLC6A3/DAT1 Antibody (NBP2-29533)Find related products by research area.
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| Gene Symbol | SLC6A3 |
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