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SLC34A1 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SLC34A1 Antibody [NBP2-13328] - Staining of human cell line HEL shows localization to nuclear speckles, plasma membrane & mitotic spindle. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SLC34A1 Antibody [NBP2-13328] - Staining of human stomach shows no positivity in glandular cells as expected.
Immunohistochemistry: SLC34A1 Antibody [NBP2-13328] - Immunohistochemical analysis of the apical (closed arrows) and intracellular (open arrows) NaPi-2a (SLC34A1) expression in the kidney. Image collected and cropped by ...read more
Immunohistochemistry-Paraffin: SLC34A1 Antibody [NBP2-13328] - Staining of human kidney shows moderate cytoplasmic and luminal membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: SLC34A1 Antibody [NBP2-13328] - Staining of human placenta shows no positivity in trophoblastic cells as expected.
Immunohistochemistry-Paraffin: SLC34A1 Antibody [NBP2-13328] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemical analysis of the apical (closed arrows) and intracellular (open arrows) NaPi-2a expression in the kidney.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SLC34A1 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: PLPVPGGHVMRGTAFAYVPSPQVLHRIPGTSAYAFPSLGPVALAEHTCPCGEVLERHEPLPAKLALEEEQKPESRLVPKLRQA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLC34A1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot Reported in (PMID: 37746883)
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100
Control Peptide
SLC34A1 Protein (NBP2-13328PEP)
Publications
Read Publications using
NBP2-13328 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26751835), Rat reactivity reported in scientific literature (PMID: 25790436).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SLC34A1 Antibody

  • FRTS2
  • Na(+)/Pi cotransporter 2A
  • Na(+)-dependent phosphate cotransporter 2A
  • naPi-2a
  • NAPI-3
  • NPT2NPHLOP1
  • NPTIIa
  • renal sodium-dependent phosphate transporter
  • SLC11
  • SLC17A2Na+-phosphate cotransporter type II
  • Sodium/phosphate cotransporter 2A
  • sodium/phosphate co-transporter
  • sodium-dependent phosphate transport protein 2A
  • Sodium-phosphate transport protein 2A
  • solute carrier family 17 (sodium phosphate), member 2
  • solute carrier family 34 (sodium phosphate), member 1
  • Solute carrier family 34 member 1

Background

The SLC34A1 gene encodes a member of the type II sodium-phosphate cotransporter family. Mutations in this gene are associated with hypophosphatemia nephrolithiasis/osteoporosis 1. Alternative splicing results in multiple transcript variants. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC34A1 Antibody (NBP2-13328)(10)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IF/IHC
(1)
IHC-P
(3)
WB
(4)
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Human
(1)
Mouse
(4)
Rat
(3)
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Showing Publications 1 - 10 of 10.
Publications using NBP2-13328 Applications Species
Feger M, Alber J, Strotmann J et al. Short-term fasting of mice elevates circulating fibroblast growth factor 23 (FGF23) Acta physiologica (Oxford, England) 2023-09-25 [PMID: 37746883] (WB, Rat) WB Rat
Huang B, Zeng Z, Li H et al. Modeling kidney development, disease, and plasticity with clonal expandable nephron progenitor cells and nephron organoids bioRxiv 2023-05-26 [PMID: 37293038] (ICC/IF, Human) ICC/IF Human
Shin S, Boadi EA, Bandyopadhyay BC Ablation of TRPC3 compromises bicarbonate and phosphate transporter activity in mice proximal tubular cells Clinical and experimental pharmacology & physiology 2022-11-26 [PMID: 36433745]
Balzer MS, Doke T, Yang YW et al. Single-cell analysis highlights differences in druggable pathways underlying adaptive or fibrotic kidney regeneration Nature communications 2022-07-11 [PMID: 35821371] (IHC-P, Mouse) IHC-P Mouse
Maruyama H, Taguchi A et al. Low bone mineral density due to secondary hyperparathyroidism in the GlatmTg(CAG-A4GALT) mouse model of Fabry disease. FASEB Bioadv 2020-01-06 [PMID: 32617522] (IF/IHC, WB, Mouse) IF/IHC, WB Mouse
Ter Braake AD, Smit AE, Bos C, et al. Magnesium prevents vascular calcification in Klotho deficiency Kidney Int. 2019-11-02 [PMID: 31866113] (WB, Mouse) WB Mouse
Mace ML, Gravesen E, Nordholm A et al. Kidney fibroblast growth factor 23 does not contribute to elevation of its circulating levels in uremia. Kidney Int. 2017-03-21 [PMID: 28341272] (WB, Rat) WB Rat
Suzuki T, Seki S, Hiramoto K et al. Hyperactivation of Nrf2 in early tubular development induces nephrogenic diabetes insipidus. Nat Commun. 2017-02-24 [PMID: 28233855]
Liu ES, Martins JS, Raimann A et al. 1,25-Dihydroxyvitamin D Alone Improves Skeletal Growth, Microarchitecture and Strength in a Murine Model of XLH, Despite Enhanced FGF23 Expression. J. Bone Miner. Res. 2016-01-11 [PMID: 26751835] (IHC-P, Mouse) IHC-P Mouse
Robijn S, Vervaet BA, D'Haese PC, Verhulst A. Evaluation of intestinal phosphate binding to improve the safety profile of oral sodium phosphate bowel cleansing. PLoS ONE. 2015-03-20 [PMID: 25790436] (IHC-P, Rat) IHC-P Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for SLC34A1 Antibody (NBP2-13328)

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Blogs on SLC34A1.

SLC34A1 - major regulator of inorganic phosphate (Pi) homeostasis
SLC34A1 encodes the 69 kDa sodium-dependent phosphate transport protein 2A (Npt2a). SLC34A is a member of the type II sodium-phosphate co-transporter family, along with SLC34A3 which encodes Npt2c. These proteins are abundantly expressed along the...  Read full blog post.

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Bioinformatics

Gene Symbol SLC34A1