![Western Blot: SLC25A6 Antibody [NBP2-20393] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A6 antibody (NBP2-20393) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SLC25A6-Antibody-Western-Blot-NBP2-20393-img0012.jpg "Western Blot: SLC25A6 Antibody [NBP2-20393] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SLC25A6 antibody (NBP2-20393) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, Rt, Po, Bv, Ch, Sh, ZeSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human SLC25A6. The exact sequence is proprietary. |
| Localization | Mitochondrion inner membrane |
| Predicted Species | Porcine (96%), Bovine (97%), Zebrafish (92%), Chicken (93%), Sheep (97%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SLC25A6 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 33 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:500.Blue: Fluoroshield with DAPI.Scale bar= 10 um.](http://images.novusbio.com/fullsize/SLC25A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20393-img0013.jpg "Immunocytochemistry/Immunofluorescence: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:500.Blue: Fluoroshield with DAPI.Scale bar= 10 um.")
![Immunocytochemistry/Immunofluorescence: SLC25A6 Antibody [NBP2-20393] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A6 protein stained by SLC25A6 antibody diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/SLC25A6-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20393-img0004.jpg "Immunocytochemistry/Immunofluorescence: SLC25A6 Antibody [NBP2-20393] - MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SLC25A6 protein stained by SLC25A6 antibody diluted at 1:500.Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - Paraffin-embedded human colon cancer. SLC25A6 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SLC25A6-Antibody-Immunohistochemistry-Paraffin-NBP2-20393-img0005.jpg "Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - Paraffin-embedded human colon cancer. SLC25A6 antibody diluted at 1:500.")
![Immunoprecipitation: SLC25A6 Antibody [NBP2-20393] - Immunoprecipitation of SLC25A6 protein from 293T whole cell extracts using 5 ug of SLC25A6 antibody (NBP2-20393). Western blot analysis was performed using SLC25A6 antibody (NBP2-20393). EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/SLC25A6-Antibody-Immunoprecipitation-NBP2-20393-img0010.jpg "Immunoprecipitation: SLC25A6 Antibody [NBP2-20393] - Immunoprecipitation of SLC25A6 protein from 293T whole cell extracts using 5 ug of SLC25A6 antibody (NBP2-20393). Western blot analysis was performed using SLC25A6 antibody (NBP2-20393). EasyBlot anti-Rabbit IgG was used as a secondary reagent.")
![Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20393_rabbit-polyclonal-slc25a6-antibody-910202420131219.jpg "Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20393_rabbit-polyclonal-slc25a6-antibody-91020242014670.jpg "Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20393_rabbit-polyclonal-slc25a6-antibody-910202420134014.jpg "Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse heart.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat muscle.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-20393_rabbit-polyclonal-slc25a6-antibody-91020242041281.jpg "Immunohistochemistry-Paraffin: SLC25A6 Antibody [NBP2-20393] - SLC25A6 antibody detects SLC25A6 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat muscle.SLC25A6 stained by SLC25A6 antibody (NBP2-20393) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")