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SKIV2L2 Antibody

Images

 
Western Blot: SKIV2L2 Antibody [NBP1-84995] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: SKIV2L2 Antibody [NBP1-84995] - Staining of human cell line U-2 OS shows positivity in nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NBP1-84995] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Western Blot: SKIV2L2 Antibody [NBP1-84995] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NBP1-84995] - Staining of human skin shows moderate nuclear positivity in epidermal cells.
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NBP1-84995] - Staining of human duodenum shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NBP1-84995] - Staining of human endometrium shows moderate nuclear positivity in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SKIV2L2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LVVDENGDFREDNFNTAMQVLRDAGDLAKGDQKGRKGGTKGPSNVFKIVKMIMERNFQPVIIFSFSKKDCEAYALQMTK
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MTREX
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Theoretical MW
118 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SKIV2L2 Protein (NBP1-84995PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SKIV2L2 Antibody

  • ATP-dependent helicase SKIV2L2
  • Dob1
  • EC 3.6.1
  • EC 3.6.4.13
  • fSAP118
  • KIAA0052functional spliceosome-associated protein 118
  • MGC142069
  • Mtr4superkiller viralicidic activity 2-like 2
  • superkiller viralicidic activity 2-like 2 (S. cerevisiae)

Background

SKIV2L2 may be involved in pre-mRNA splicing

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol MTREX
Entrez
Uniprot