![Western Blot: SKIV2L2 Antibody [NB100-1574] - Analysis of SKIV2L2 in HeLa cell lysates treated with (1) si-control and (2) SKIV2L2 siRNA using anti-SKIV2L2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/SKIV2L2-Antibody-Western-Blot-NB100-1574-img0010.jpg "Western Blot: SKIV2L2 Antibody [NB100-1574] - Analysis of SKIV2L2 in HeLa cell lysates treated with (1) si-control and (2) SKIV2L2 siRNA using anti-SKIV2L2 antibody. Image from verified customer review.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP, ICC/IF, KD |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human SuperKiller Viralicidic Activity 2-Like 2 using the numbering given in entry NP_056175.2 (GeneID 23517). |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | MTREX |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | For WB: When blotting for mouse SKIV2L2, lower dilutions of antibody and/or longer exposure times may be required. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. SKIV2L2 antibody validated for WB from a verified customer review. |
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| Theoretical MW | 118 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | TBS and 0.1% BSA |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Immunogen affinity purified |
| Images | Ratings | Applications | Species | Date | Details | ||||||||
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reviewed by:
Verified Customer |
WB | Human | 05/05/2015 |
Summary
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Secondary Antibodies |
Isotype Controls |
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![Western Blot: SKIV2L2 Antibody [NB100-1574] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiSKIV2L2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SKIV2L2-Antibody-Western-Blot-NB100-1574-img0012.jpg "Western Blot: SKIV2L2 Antibody [NB100-1574] - Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiSKIV2L2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NB100-1574] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SKIV2L2-Antibody-Immunohistochemistry-Paraffin-NB100-1574-img0013.jpg "Immunohistochemistry-Paraffin: SKIV2L2 Antibody [NB100-1574] - FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml). Detection: DAB")
![SKIV2L2 Antibody [NB100-1574] - Detection of human SKIV2L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody NB100-1574 used for IP at 3 ug per reaction. SKIV2L2 was also immunoprecipitated by rabbit anti-SKIV2L2 antibody NB100-1575. For blotting immunoprecipitated SKIV2L2, NB100-1574 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/SKIV2L2-Antibody-NB100-1574-img0014.jpg "SKIV2L2 Antibody [NB100-1574] - Detection of human SKIV2L2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SKIV2L2 antibody NB100-1574 used for IP at 3 ug per reaction. SKIV2L2 was also immunoprecipitated by rabbit anti-SKIV2L2 antibody NB100-1575. For blotting immunoprecipitated SKIV2L2, NB100-1574 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![SKIV2L2 Antibody [NB100-1574] - Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml).](http://images.novusbio.com/fullsize/SKIV2L2-Antibody-NB100-1574-img0015.jpg "SKIV2L2 Antibody [NB100-1574] - Affinity purified rabbit anti-SKIV2L2 used at a dilution of 1:200 (1ug/ml).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")
