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Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Name: Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-80300
Price: 699 USD

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ELISA: Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80300] - Samples were spiked with high concentrations of Human Sirtuin 1/SIRT1 and diluted with Reference Standard & Sample Diluent to produce samples with ...read more

ELISA: Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) [NBP2-80300] - Standard Curve Reference

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	ELISA
Suitable Sample Type	Serum, plasma and other biological fluids
Standard Curve Range	0.313-20 ng/mL
Sensitivity	0.188 ng/mL

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Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) Summary

Specificity	This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed.
Standard Curve Range	0.313-20 ng/mL
Sensitivity	0.188 ng/mL
Assay Type	Sandwich-ELISA
Inter-Assay	CV% < 4.59%
Intra-Assay	CV% < 5.18%
Spike Recovery	86-106%
Sample Volume	100 ul
Kit Type	ELISA Kit (Colorimetric)
Gene	SIRT1

Applications/Dilutions

Dilutions

ELISA

Packaging, Storage & Formulations

Storage

Storage of components varies. See protocol for specific instructions.

Kit Components

Components

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25X)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Alternate Names for Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

EC 3.5.1
S. cerevisiae, homolog) 1
SIR2alpha
SIR2L1
SIR2-like protein 1
SIRT1
Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) Manual

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Bioinformatics

Gene Symbol	SIRT1
Uniprot	Q96EB6(Human) Q96EB6(Human)

Popular Products	Company Information	Support	Stay Connected
Antibodies Secondary Antibodies Isotype Controls Proteins and Peptides ELISA Kits Sample Size New Products	About Us Careers Novus Guarantee Contact Us Our Team	Technical Support FAQs Help Finding Antibodies Protocols

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Species Glossary

Images

Product Details

Order Details

Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric) Summary

Applications/Dilutions

Packaging, Storage & Formulations

Kit Components

Alternate Names for Human Sirtuin 1/SIRT1 ELISA Kit (Colorimetric)

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Sirtuin 1/SIRT1 ELISA Kit (NBP2-80300) (0)

Reviews for Sirtuin 1/SIRT1 ELISA Kit (NBP2-80300) (0)

Product General Protocols

FAQs for Sirtuin 1/SIRT1 ELISA Kit (NBP2-80300) (0)

Additional Sirtuin 1/SIRT1 Products

Research Areas for Sirtuin 1/SIRT1 ELISA Kit (NBP2-80300)

Blogs on Sirtuin 1/SIRT1

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Bioinformatics