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SH3BP1 Antibody

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Western Blot: SH3BP1 Antibody [NB100-1398] - SH3BP1 in Human Brain lysate using NB100-1398 at 1 ug/ ml.
Immunohistochemistry-Paraffin: SH3BP1 Antibody [NB100-1398] - NB100-1398 (0.3ug/ml) staining of paraffin embedded Human Kidnay. Cytoplasmic staining of lymphocytes A) in the interstitial parenchyma of the kidney. B) in ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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SH3BP1 Antibody Summary

Immunogen
Peptide with sequence C-PQPRPRSLASETN corresponding to C-Terminus according to NP_061830.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SH3BP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.3 ug/ml
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
Application Notes
ICC successful usage in Caco-2 cells is reported in scientific literature (Elbediwy et al, J.Biol. Chem, 2012). WB: Approx. 70 kDa band observed in human lung and kidney lysates (calculated MW of 75.7 kDa band according to NP_061830.3). IHC-P: Human kidney stains the cytoplasm of lymphoid cells in the interstitial parenchyma and of lymphocytes in a glomerulus.
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
SH3BP1 Overexpression Lysate
Control Peptide
SH3BP1 Peptide (NB100-1398PEP)
Publications
Read Publication using NB100-1398.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SH3BP1 Antibody

  • 3BP-1
  • ARHGAP43
  • SH3 domain binding protein 1
  • SH3 domain-binding protein 1
  • SH3-domain binding protein 1

Background

Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. This protein binds preferentially to c-Abl proto-oncogene, SRC and GRB2. Shows GAP activity for Rac-related proteins but not for Rho- or Ras-related proteins. It inhibits PDGF-induced membrane ruffling mediated by Rac (By similarity).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SH3BP1 Antibody (NB100-1398)(1)


Showing Publication 1 - 1 of 1.
Publication using NB100-1398 Applications Species
Dunham I, Shimizu N, Roe BA et al. The DNA sequence of human chromosome 22. Nature 1999-12-02 [PMID: 10591208]

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Bioinformatics

Gene Symbol SH3BP1
Entrez
Uniprot