![Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Kelly (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-129202311344711.jpg "Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Kelly (A) and K562 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Rt, Po, BvSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence C-TMNTSGHDFEEL corresponding to C-Terminus according to NP_005016.1. |
| Epitope | C Terminus |
| Specificity | Reported variants represent identical protein (NP_005016.1; NP_001116224.1). |
| Predicted Species | Porcine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SERPINI1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Western blot: Approx 48kDa band observed in lysates of cell lines Kelly and K562 and in Human Placenta and Mouse and Rat Brain lysates (calculated MW of 46.4kDa according to Human NP_005016.1, Mouse NP_033276.1 and Rat NP_446231.1). Primary incubation 1 hour at room temperature. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
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| Publication using NB100-1529 | Applications | Species |
|---|---|---|
| Onda M, Belorgey D, Sharp LK, Lomas DA. Latent Ser49Pro neuroserpin forms polymers in the dementia familial encephalopathy with neuroserpin inclusion bodies. J Biol Chem. 2005-01-21 [PMID: 15664988] |
Secondary Antibodies |
Isotype Controls |
Research Areas for Serpin I1/Neuroserpin Antibody (NB100-1529)Find related products by research area.
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| Gene Symbol | SERPINI1 |
| Entrez |
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![Flow Cytometry: Serpin I1/Neuroserpin Antibody [NB100-1529] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134471.jpg "Flow Cytometry: Serpin I1/Neuroserpin Antibody [NB100-1529] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Human Placenta (A), Mouse Brain (B) and (0.5ug/ml) Rat Braun (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134479.jpg "Western Blot: Serpin I1/Neuroserpin Antibody [NB100-1529] - (1ug/ml) staining of Human Placenta (A), Mouse Brain (B) and (0.5ug/ml) Rat Braun (C) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Serpin I1/Neuroserpin Antibody [NB100-1529] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nb100-1529_goat-polyclonal-serpin-i1-neuroserpin-antibody-12920231134477.jpg "Immunohistochemistry-Paraffin: Serpin I1/Neuroserpin Antibody [NB100-1529] - (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
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![Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.](https://images.novusbio.com/images/Human-Brain-Whole-Tissue-Lysate-Adult-Normal-Western-Blot-NB820-59177-img0001.jpg "Western Blot: Human Brain Whole Tissue Lysate (Adult Normal) [NB820-59177] - Beta Actin expression on human brain whole tissue lysate using anti-Beta Actin antibody (Cat. # NBP1-47423). Image from verified customer review.")
