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Serpin E1/PAI-1 Antibody

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Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of A549 (A), HepG2 (B) and HeLa (C) lysates (35 ug protein in RIPA buffer).Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by ...read more
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of paraffin embedded Human Placenta. Antibody at 4 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Western Blot: Serpin E1/PAI-1 Antibody [NB100-1498] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Serpin E1/PAI-1 Antibody Summary

Immunogen
Peptide with sequence C-GFKIDDKGMAPALRH corresponding to internal region according to NP_000593.1, NP_001158885.1.
Specificity
This antibody is expected to recognize both reported isoforms.
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SERPINE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 4 - 6 ug/mL
  • Immunohistochemistry-Paraffin 4-6 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/mL
Publications
Read Publications using NB100-1498.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Serpin E1/PAI-1 Antibody

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type palsminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin E1/PAI-1 Antibody (NB100-1498)(2)

We have publications tested in 1 application: ELISA.


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(1)
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Bioinformatics

Gene Symbol SERPINE1
Entrez
Uniprot