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Septin-4 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SEPT4 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Septin-4 Antibody [NBP1-90093] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human testis shows moderate cytoplasmic positivity in spermatids.
Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Septin-4 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQE
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SEPTIN4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Septin-4 Protein (NBP1-90093PEP)
Publications
Read Publications using
NBP1-90093 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Septin-4 Antibody

  • Apoptosis-related protein in the TGF-beta signaling pathway
  • ARTSPeanut-like protein 2
  • Bradeion beta
  • BRADEION
  • Brain protein H5
  • CE5B3
  • Cell division control-related protein 2
  • Cerebral protein 7
  • H5
  • hCDCREL-2SEP4
  • hucep-7
  • MART
  • peanut-like 2 (Drosophila)
  • peanut-like 2
  • PNUTL2CE5B3 beta
  • septin 4
  • septin-4
  • septin-M

Background

Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-b signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-b and, to a lesser extent, by other apoptotic agents, such as TNF-a and Fas ligand.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Septin-4 Antibody (NBP1-90093)(3)

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on Septin-4

There are no specific blogs for Septin-4, but you can read our latest blog posts.
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Bioinformatics

Gene Symbol SEPTIN4