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![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SEPT4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SEPT4 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Septin-4 Antibody [NBP1-90093] - Staining of human cell line Hep G2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90093-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0012.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0010.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human testis shows moderate cytoplasmic positivity in spermatids.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0011.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90093] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90093-img0013.jpg)
![Western Blot: Septin-4 Antibody [NBP1-90094] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-90094-img0004.jpg "Western Blot: Septin-4 Antibody [NBP1-90094] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90094] - Staining in human cerebral cortex and pancreas tissues using anti-SEPT4 antibody. Corresponding SEPT4 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90094-img0007.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90094] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer and distinctly stained neuropil.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90094-img0002.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90094] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90094-img0005.jpg)
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-90094] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-90094-img0006.jpg)
![Western Blot: Septin-4 Antibody [NBP1-52095] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-52095-img0003.jpg "Western Blot: Septin-4 Antibody [NBP1-52095] - Staining of Human Heart lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-52095] - Staining of paraffin embedded Human Cerebellum. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-52095-img0005.jpg)
![Flow Cytometry: Septin-4 Antibody [NBP1-52095] - Staining of paraffin embedded Human Cerebellum with antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Flow-Cytometry-NBP1-52095-img0006.jpg)
![Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-52096-img0002.jpg "Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Septin-4 Antibody [NBP1-52096] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Flow-Cytometry-NBP1-52096-img0003.jpg)
![Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of Jurkat nuclear (A) and LNCaP (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-52096-img0001.jpg)
![Western Blot: Septin-4 Antibody [NBP1-76667] - Human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with Septin-4 antibody at 2 ug /ml.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP1-76667-img0003.jpg "Western Blot: Septin-4 Antibody [NBP1-76667] - Human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with Septin-4 antibody at 2 ug /ml.")
![Immunohistochemistry-Paraffin: Septin-4 Antibody [NBP1-76667] - Human lung tissue with ARTS antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-76667-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Septin-4 Antibody [NBP1-76667] - Human Lung cells with ARTS antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Immunofluorescence-NBP1-76667-img0004.jpg)
![Western Blot: Septin-4 Antibody [NBP3-12491] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 54 KDa.](http://images.novusbio.com/fullsize/Septin-4-Antibody-Western-Blot-NBP3-12491-img0001.jpg "Western Blot: Septin-4 Antibody [NBP3-12491] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 54 KDa.")
![SDS-Page: Recombinant Human Septin-4 Protein [H00005414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00005414-P01-1.jpg "SDS-Page: Recombinant Human Septin-4 Protein [H00005414-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Septin-4, variant 2 Overexpression Lysate (Adult Normal) [NBL1-07143] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 2.](http://images.novusbio.com/fullsize/Septin-4-variant-2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07143-img0002.jpg "Western Blot: Septin-4, variant 2 Overexpression Lysate (Adult Normal) [NBL1-07143] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 2.")
![Western Blot: Septin-4, variant 1 Overexpression Lysate (Adult Normal) [NBL1-07142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 1.](http://images.novusbio.com/fullsize/Septin-4-variant-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07142-img0002.jpg "Western Blot: Septin-4, variant 1 Overexpression Lysate (Adult Normal) [NBL1-07142] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 1.")
![Western Blot: Septin-4, variant 3 Overexpression Lysate (Adult Normal) [NBL1-07144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 3.](http://images.novusbio.com/fullsize/Septin-4-variant-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-07144-img0002.jpg "Western Blot: Septin-4, variant 3 Overexpression Lysate (Adult Normal) [NBL1-07144] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Septin-4, variant 3.")