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RhoC Antibody (7X5I9)

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Western Blot: RhoC Antibody (7X5I9) [NBP3-16333] - Western blot analysis of extracts of various cell lines, using RhoC Rabbit mAb (NBP3-16333) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333] - Immunofluorescence analysis of NIH-3T3 cells using RhoC Rabbit mAb (NBP3-16333) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: RhoC Antibody (7X5I9) [NBP3-16333] - Immunohistochemistry of paraffin-embedded rat testis using RhoC Rabbit mAb (NBP3-16333) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [NBP3-16333] - Immunofluorescence analysis of NIH-3T3 cells using RhoC Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: RhoC Antibody (7X5I9) [RhoC] - Immunofluorescence analysis of NIH-3T3 cells using RhoC Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
7X5I9
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

RhoC Antibody (7X5I9) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human RhoC (P08134). MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYIADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRQDEHTRRELAKMKQEPVRSEEGRDMANRISAFGYLECSAKTKEGVREVFEMATRAGLQVRKNKRRRGCPIL
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RHOC
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RhoC Antibody (7X5I9)

  • ARH9ARHCRho cDNA clone 9
  • H9
  • MGC1448
  • MGC61427
  • oncogene RHO H9
  • ras homolog gene family, member C
  • RAS-related homolog 9
  • rhoC GTPase
  • RhoC
  • RHOH9
  • rho-related GTP-binding protein RhoC
  • small GTP binding protein RhoC

Background

RhoC encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RhoC Antibody (NBP3-16333) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for RhoC Antibody (NBP3-16333) (0)

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Secondary Antibodies

 

Isotype Controls

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Research Areas for RhoC Antibody (NBP3-16333)

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Blogs on RhoC.

Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.

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Bioinformatics

Gene Symbol RHOC