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SEPHS2 Antibody (2G9)

Images

 
Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02] - SEPHS2 monoclonal antibody (M02), clone 2G9 Analysis of SEPHS2 expression in K-562.
Immunohistochemistry-Paraffin: SEPHS2 Antibody (2G9) [H00022928-M02] - Analysis of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.
Sandwich ELISA: SEPHS2 Antibody (2G9) [H00022928-M02] - Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clone
2G9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SEPHS2 Antibody (2G9) Summary

Immunogen
SEPHS2 (NP_036380.2, 61 a.a. ~ 150 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GCKVPQEALLKLLAGLTRPDVRPPLGRGLVGGQEEASQEAGLPAGAGPSPTFPALGIGMDSCVIPLRHGGLSLVQTTDFFYPLVEDPYMM
Specificity
SEPHS2 - selenophosphate synthetase 2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SEPHS2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SEPHS2 Antibody (2G9)

  • EC 2.7.9.3
  • Selenophosphate synthase 2
  • selenophosphate synthetase 2
  • SPS2water dikinase 2

Background

This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem-loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop. Alternatively spliced transcripts have been identified, but their biological validity has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SEPHS2
Entrez
OMIM
Uniprot