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![Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02] - SEPHS2 monoclonal antibody (M02), clone 2G9 Analysis of SEPHS2 expression in K-562.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-2G9-Western-Blot-H00022928-M02-img0007.jpg "Western Blot: SEPHS2 Antibody (2G9) [H00022928-M02] - SEPHS2 monoclonal antibody (M02), clone 2G9 Analysis of SEPHS2 expression in K-562.")
![Immunohistochemistry-Paraffin: SEPHS2 Antibody (2G9) [H00022928-M02] - Analysis of monoclonal antibody to SEPHS2 on formalin-fixed paraffin-embedded human colon. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-2G9-Immunohistochemistry-Paraffin-H00022928-M02-img0005.jpg)
![Sandwich ELISA: SEPHS2 Antibody (2G9) [H00022928-M02] - Detection limit for recombinant GST tagged SEPHS2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-2G9-Sandwich-ELISA-H00022928-M02-img0006.jpg)
![Western Blot: SEPHS2 Antibody [NBP1-77776] - Shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Western-Blot-NBP1-77776-img0002.jpg "Western Blot: SEPHS2 Antibody [NBP1-77776] - Shows detection of SPS2 in NIH3T3 cells over-expressing this protein. No signal is seen in control lysates or in lysates from cells over-expressing the protein after pre-treatment with SPS2 siRNA. Endogenous SPS2 can be detected in mouse kidney, liver and testes tissue lysates. Partial cross-reactivity is seen against recombinant SPS1. The primary antibody was used at a 1:1000 dilution. Personal Communication, D. Hatfield, NCI, Bethesda, MD.")
![Immunocytochemistry/Immunofluorescence: SEPHS2 Antibody [NBP2-13293] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13293-img0002.jpg "Immunocytochemistry/Immunofluorescence: SEPHS2 Antibody [NBP2-13293] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.")
![Immunohistochemistry-Paraffin: SEPHS2 Antibody [NBP2-13293] - Staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-13293-img0001.jpg)
![Western Blot: SEPHS2 Antibody [NBP1-32645] - (30 ug whole cell lysate) HeLa S3 7.5% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Western-Blot-NBP1-32645-img0001.jpg "Western Blot: SEPHS2 Antibody [NBP1-32645] - (30 ug whole cell lysate) HeLa S3 7.5% SDS PAGE diluted at 1:1000")
![Immunocytochemistry/Immunofluorescence: SEPHS2 Antibody [NBP1-32645] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32645-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SEPHS2 Antibody [NBP2-97672] - Immunofluorescence staining of SEPHS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SEPHS2 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97672-img0001.jpg "Immunocytochemistry/Immunofluorescence: SEPHS2 Antibody [NBP2-97672] - Immunofluorescence staining of SEPHS2 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human SEPHS2 polyclonal antibody (dilution ratio 1:100) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Nucleus.")
![Immunohistochemistry-Paraffin: SEPHS2 Antibody [NBP2-97672] - Immunochemical staining of human SEPHS2 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/SEPHS2-Antibody-Immunohistochemistry-Paraffin-NBP2-97672-img0002.jpg)
![Western Blot: SEPHS2 Overexpression Lysate (Adult Normal) [NBP2-04332] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEPHS2.](http://images.novusbio.com/fullsize/SEPHS2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04332-img0001.jpg "Western Blot: SEPHS2 Overexpression Lysate (Adult Normal) [NBP2-04332] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEPHS2.")