![Western Blot: Semaphorin 3F Antibody [NBP3-23386] - Western blot analysis of Semaphorin 3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Semaphorin 3F (NBP3-23386) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23386_rabbit-semaphorin-3f-pab-19920231410143.jpg "Western Blot: Semaphorin 3F Antibody [NBP3-23386] - Western blot analysis of Semaphorin 3F expression in adult mouse brain (lane 1), kidney (lane 2), and liver (lane 3). The blot was probed with anti-Semaphorin 3F (NBP3-23386) at 1:1000.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | Stable for at least 1 year at -20°C. |
| Immunogen | Semaphorin 3F synthetic peptide (coupled to carrier protein) corresponds to amino acids from the C-terminal region of human Sema-3F. This sequence is highly conserved in rat and mouse Sema-3F, and has low homology to other semaphorin family members. [UniProt# Q13275] |
| Specificity | The antibody detects a 95 kDa protein corresponding to the apparent molecular mass of Semaphorin 3F on SDS-PAGE immunoblots of adult mouse brain and liver. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEMA3F |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 95 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. |
| Buffer | PBS, 1 mg/ml BSA and 50% glycerol |
| Preservative | 0.05% Sodium Azide |
| Purity | Antigen Affinity-purified |
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21496.jpg)
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21497.jpg)
![Western Blot Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Western_Blot_21557.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | SEMA3F |
![Immunohistochemistry Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Immunohistochemistry_16084.jpg)
![Western Blot Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Western_Blot_20048.jpg)
![Simple Western Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Simple_Western_20049.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3A_1250S3_1473.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunohistochemistry Plexin A3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A3_AF4075_Immunohistochemistry_11308.jpg)
![Immunohistochemistry Plexin A3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af4075_mouse-rat-plexin-a3-affinity-purified-polyclonal-ab-immunohistochemistry-1642021131527.jpg)
![Immunocytochemistry Semaphorin 3C Antibody (238835) [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3C_MAB1728_Immunocytochemistry_11554.jpg)
![Immunohistochemistry Semaphorin 3C Antibody (238835) [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3C_MAB1728_Immunohistochemistry_19277.jpg)
![Immunohistochemistry Semaphorin 6A Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1615_mouse-semaphorin-6a-affinity-purified-polyclonal-ab-immunohistochemistry-92202314926.jpg)
![Immunohistochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunohistochemistry_10417.jpg)
![Immunocytochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunocytochemistry_11095.jpg)
![Western Blot Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Western_Blot_9601.jpg)
![Immunohistochemistry Semaphorin 3E Antibody [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3E_AF3239_Immunohistochemistry_11300.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")