![Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Positive control: Lane1 : No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F Primary, Antibody Dilution: 1 ug/ml Secondary Antibody: Anti-AP Secondry, Antibody Dilution: 1 : 1000.](http://images.novusbio.com/fullsize/Semaphorin-3F-Antibody-Western-Blot-NBP1-91480-img0005.jpg "Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Positive control: Lane1 : No transfection, Lane, 2: Cell lysates from HEK293 cells were transfected with alkaline phosphatase (AP-) tagged Sema3F, Lane3: AP-alone, Lane4: The cell media from cells transfected with AP-Sema3F Primary, Antibody Dilution: 1 ug/ml Secondary Antibody: Anti-AP Secondry, Antibody Dilution: 1 : 1000.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, IHC, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptide directed towards the N terminal of human Sema3f. Peptide sequence FIHAELIPDSAERNDDKLYFFFRERSAEAPQNPAVYARIGRICLNDDGGH. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | SEMA3F |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21496.jpg)
![Immunohistochemistry Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Immunohistochemistry_21497.jpg)
![Western Blot Neuropilin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin1_AF566_Western_Blot_21557.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480] - Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type: 1. 5mL conditioned media from HEK293F cells transfected with 1 : Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP.](http://images.novusbio.com/fullsize/Semaphorin-3F-Antibody-Immunohistochemistry-NBP1-91480-img0004.jpg "Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480] - Researcher: Dr. Chrisna LeVaillant, School of Anatomy and Human BiologyApplication: IPSpecies+tissue/cell type: 1. 5mL conditioned media from HEK293F cells transfected with 1 : Sema 3F_AP, 2: Sema3C_FLAG, 3: UntransfectedAmount of IP.")
![Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Titration: 1.0 ug/ml Positive Control: Mouse Liver.](http://images.novusbio.com/fullsize/Semaphorin-3F-Antibody-Western-Blot-NBP1-91480-img0002.jpg "Western Blot: Semaphorin 3F Antibody [NBP1-91480] - Titration: 1.0 ug/ml Positive Control: Mouse Liver.")
![Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480] - Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution: 1 : 500 Color/Signal Descriptions: Gene name: SEMA3F.](http://images.novusbio.com/fullsize/Semaphorin-3F-Antibody-Immunohistochemistry-NBP1-91480-img0003.jpg "Immunohistochemistry: Semaphorin 3F Antibody [NBP1-91480] - Untransfected HEK293 and Sema3F-AP transfected HEK293 Primary Antibody Dilution: 1 : 1000 Secondary Antibody: Anti rabbit-Alexa Fluor 488 Secondary Antibody Dilution: 1 : 500 Color/Signal Descriptions: Gene name: SEMA3F.")
![Immunohistochemistry Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Immunohistochemistry_16084.jpg)
![Western Blot Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Western_Blot_20048.jpg)
![Simple Western Neuropilin-2 Antibody [Unconjugated]](https://images.novusbio.com/images2/Neuropilin2_AF567_Simple_Western_20049.jpg)
![Bioactivity Semaphorin 3A [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3A_1250S3_1473.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![Immunohistochemistry Plexin A3 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A3_AF4075_Immunohistochemistry_11308.jpg)
![Immunohistochemistry Plexin A3 Antibody [Unconjugated]](https://images.novusbio.com/images2/af4075_mouse-rat-plexin-a3-affinity-purified-polyclonal-ab-immunohistochemistry-1642021131527.jpg)
![Immunocytochemistry Semaphorin 3C Antibody (238835) [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3C_MAB1728_Immunocytochemistry_11554.jpg)
![Immunohistochemistry Semaphorin 3C Antibody (238835) [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3C_MAB1728_Immunohistochemistry_19277.jpg)
![Immunohistochemistry Semaphorin 6A Antibody [Unconjugated]](https://images.novusbio.com/images/antibody/af1615_mouse-semaphorin-6a-affinity-purified-polyclonal-ab-immunohistochemistry-92202314926.jpg)
![Immunohistochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunohistochemistry_10417.jpg)
![Immunocytochemistry Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Immunocytochemistry_11095.jpg)
![Western Blot Plexin A1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Plexin_A1_AF4309_Western_Blot_9601.jpg)
![Western Blot Plexin A4 Antibody (707201) [Unconjugated]](https://images.novusbio.com/images2/Plexin_A4_MAB5856_Western_Blot_11255.jpg)
![Simple Western Plexin A4 Antibody (707201) [Unconjugated]](https://images.novusbio.com/images2/mab5856_human-mouse-rat-plexin-a4-mab-clone-707201-simple-western-2682022154250.jpg)
![Immunohistochemistry Semaphorin 3E Antibody [Unconjugated]](https://images.novusbio.com/images2/Semaphorin_3E_AF3239_Immunohistochemistry_11300.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")