SCP1 Antibody

SCP1 (synaptonemal complex protein 1), also called cancer/testis antigen 8 (CT8), belongs to a growing family of cancer/testis antigens (CTA) which includes several proteins (MAGE, GAGE/PAGE/XAGE, NY-ESO-1/LAGE-1, SSX, SPANX, TRAG-3, BAGE, OY-TES-1, CT17, NY-BR-3 and SCP-1) that are not expressed in normal tissues except for testis and, in some cases, placenta. SCP1 is found in a complex with SYCE1 and SYCE2, and is a major component of the transverse filaments of synaptonemal complexes. SCP-1 is established to be expressed selectively during the meiotic prophase of spermatocytes formation and is involved in the pairing of homologous chromosomes during meiosis I. SCP1 is localized in tripartite segments of synaptonemal complexes, between lateral elements in the nucleus, and is found only where the chromosome cores are synapsed. Its N-terminus is found towards the center, whereas, the C-terminus extends well into the lateral domain of the synaptonemal complex. SCP-1 differs from other members of CTA family by its localization on chromosome 1 and it is frequently observed to have variable expression in neoplastic tissues/cell lines including malignant gliomas, breast, renal cell, ovarian, pancreatic and hepatocellular carcinomas. Because of exclusive expression of SCP1 in testis/placenta, it is emerging as an important target for cancer research and therapy.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.

Showing Publications 1 - 10 of 13. Show All 13 Publications. Collapse Publications.

Publications using NB300-228	Applications	Species
Miao Du, Shikun Chen, Yang Chen, Xinxu Yuan, Huansheng Dong Testicular fat deposition attenuates reproductive performance via decreased follicle-stimulating hormone level and sperm meiosis and testosterone synthesis in mouse Animal Bioscience 2024-01-01 [PMID: 37641828]
Mizuta K, Katou Y, Nakakita B et al. Ex vivo reconstitution of fetal oocyte development in humans and cynomolgus monkeys The EMBO journal 2022-08-01 [PMID: 35912849]
Liu C, Liu H, Zhang H et al. Paternal USP26 mutations raise Klinefelter syndrome risk in the offspring of mice and humans The EMBO journal 2021-05-12 [PMID: 33978233]
Wei H, Khawar MB, Tang W et al. Sirt6 is required for spermatogenesis in mice Aging (Albany NY) 2020-09-11 [PMID: 32915773]
Chien JC, Tabet E, Pinkham K et al. A multiplexed bioluminescent reporter for sensitive and non-invasive tracking of DNA double strand break repair dynamics in vitro and in vivo Nucleic Acids Res 2020-08-16 [PMID: 32797168]
Wang L, Tu Z, et al. Dual roles of TRF1 in tethering telomeres to the nuclear envelope and protecting them from fusion during meiosis. Cell Death Differ 2018-06-01 [PMID: 29311622] (ICC/IF, Mouse)	ICC/IF	Mouse
Xu Z, Song Z, Li G et al. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res 2016-07-18 [PMID: 27431324]
Liu YJ, Liu C, Chang Z et al. Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway J. Biol. Chem. 2016-02-08 [PMID: 26858254] (IHC-P, Mouse)	IHC-P	Mouse
Zheng Y, Liu Y, Jin H et al. Scavenger Receptor B1 is a Potential Biomarker of Human Nasopharyngeal Carcinoma and Its Growth is Inhibited by HDL-mimetic Nanoparticles. Theranostics 2013-06-25 [PMID: 23843895] (WB, Human)	WB	Human
Bisig CG, Guiraldelli MF, Kouznetsova A et al. Synaptonemal Complex Components Persist at Centromeres and Are Required for Homologous Centromere Pairing in Mouse Spermatocytes PLoS Genet 2012-06-01 [PMID: 22761579] (ICC/IF, Mouse)	ICC/IF	Mouse
Takada Y, Naruse C, Costa Y et al. HP1{gamma} links histone methylation marks to meiotic synapsis in mice. Development;138(19):4207-4217. 2011-10-01 [PMID: 21896631]
Yamauchi K, Hasegawa K, Chuma S et al. In vitro germ cell differentiation from cynomolgus monkey embryonic stem cells. PLoS One 2009-01-01 [PMID: 19399191] (ICC/IF, Primate)	ICC/IF	Primate
Dann, CT et al. Heritable and stable gene knockdown in rats. Proc Natl Acad Sci U S A5;103(30):11246-51. 2006-07-02 [PMID: 16844779] (ICC/IF, Rat)	ICC/IF	Rat
Show All 13 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	1 reviewed by: Verified Customer	ICC	Human	03/26/2024	View Summary Application Immunocytochemistry Sample Tested Induced pluripotent stem cells overexpressing SYCP1,Meiocytes Species Human Lot C-8 Comments Comments ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** Stained at 1:250 dilution, using donkey anti-rabbit 488 secondary antibody. No signal was observed in human meiotic cells. I also tested this with human iPSCs overexpressing SYCP1 and also observed no signal.

Array NB300-228

• SCP1 Antibodies
• SCP1 Proteins