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SART3 Antibody

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Immunocytochemistry/Immunofluorescence: SART3 Antibody [NBP1-31487] - Analysis of HeLa, using NBP1-31487 at 1:200 dilution.
Immunocytochemistry/ Immunofluorescence: SART3 Antibody [NBP1-31487] - Analysis of HeLa, using NBP1-31487 at 1:200 dilution.
Immunohistochemistry-Paraffin: SART3 Antibody [NBP1-31487] - NCI-N87 xenograft, using SART3 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: SART3 Antibody [NBP1-31487] - ART3 antibody [C3], C-term immunoprecipitates SART3 protein in IP experiments.IP samples: HepG2 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. ...read more
Western Blot: SART3 Antibody [NBP1-31487] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SART3 antibody [C3], C-term (NBP1-31487) diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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SART3 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SART3. The exact sequence is proprietary.
Localization
Cytoplasm, Nucleus speckle
Predicted Species
Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SART3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:500-1:3000
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Antigen Affinity-purified

Alternate Names for SART3 Antibody

  • DSAP1
  • hSART-3
  • KIAA0156squamous cell carcinoma antigen recognised by T cells 3
  • P100
  • p110
  • p110(nrb)
  • RP11-13G14
  • SART-3
  • squamous cell carcinoma antigen recognized by T cells 3
  • squamous cell carcinoma antigen recognized by T-cells 3
  • Tat-interacting protein of 110 kDa
  • Tip110
  • TIP110MGC138188

Background

The protein encoded by this gene is an RNA-binding nuclear protein that is a tumor-rejection antigen. This antigen possesses tumor epitopes capable of inducing HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes in cancer patients and may be useful for specific immunotherapy. This gene product is found to be an important cellular factor for HIV-1 gene expression and viral replication. It also associates transiently with U6 and U4/U6 snRNPs during the recycling phase of the spliceosome cycle. This encoded protein is thought to be involved in the regulation of mRNA splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SART3