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SALL4 Antibody (CL12183)

Images

 
Orthogonal Strategies: Analysis in human testis and endometrium tissues using NBP3-21209 antibody. Corresponding SALL4 RNA-seq data are presented for the same tissues.
Staining of mouse embryo E11 shows moderate to strong nuclear positivity in in a subset of cells in developing hindbrain.
Staining of human ovary shows strong nuclear positivity in oocyte.
Staining of human endometrium shows no positivity in glandular cells as expected.
Staining of NTERA-2 cells using the Anti-SALL4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where ...read more
Staining of human cerebellum shows no positivity in Purkinje cells as expected.
Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.
Analysis in human cell line NTERA-2.
Staining of mouse embryo E11 shows strong nuclear positivity in cells in developing testis.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL12183
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SALL4 Antibody (CL12183) Summary

Immunogen
This antibody was developed against a Synthetic Peptide of Q9UJQ4. Actual immunogen sequence is proprietary.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SALL4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Rat (85%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for SALL4 Antibody (CL12183)

  • dJ1112F19.1
  • DRRS
  • HSAL4
  • MGC133050
  • SALL4
  • sal-like 4 (Drosophila)
  • sal-like protein 4
  • Zinc finger protein 797
  • Zinc finger protein SALL4
  • ZNF797
  • ZNF797sal (Drosophila)-like 4

Background

Sall4 is encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SALL4