![Western Blot: SAH3 Antibody [NB100-401] - Samples: NETN whole cell lysate (20 ug for WB; 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-SAH3 antibody used at the indicated concentration for WB and 2 ug/mg lysate for IP. Detection: HRP conjugated goat anti-rabbit IgG used at 1:10,000 followed by a chemiluminescent substrate and exposure to film for 10 seconds.](http://images.novusbio.com/fullsize/SAH3-Antibody-Western-Blot-NB100-401-img0003.jpg "Western Blot: SAH3 Antibody [NB100-401] - Samples: NETN whole cell lysate (20 ug for WB; 1 mg for IP) from HeLa cells. Antibody: Affinity purified rabbit anti-SAH3 antibody used at the indicated concentration for WB and 2 ug/mg lysate for IP. Detection: HRP conjugated goat anti-rabbit IgG used at 1:10,000 followed by a chemiluminescent substrate and exposure to film for 10 seconds.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IP |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1.0 mg/ml |
| Immunogen | The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human adenosylhomocysteinase 3 (KIAA0828) using the numbering given in entry NP_056143.1 (GeneID 23382). |
| Localization | Cytoplasm |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | AHCYL2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This SAH3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation, and Western Blot, where a band should be seen at ~70-80 kDa. In ICC/IF, cytoplasmic staining was observed in neuro-2a cells. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Tris-Citrate/Phosphate (pH 7.0 - 8.0) |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Immunogen affinity purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | AHCYL2 |
| Entrez |
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| Uniprot |
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![Immunocytochemistry/Immunofluorescence: SAH3 Antibody [NB100-401] - SAH3 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).](http://images.novusbio.com/fullsize/SAH3-Antibody-Immunocytochemistry-Immunofluorescence-NB100-401-img0004.jpg "Immunocytochemistry/Immunofluorescence: SAH3 Antibody [NB100-401] - SAH3 antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 549 (red).")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")