![Western Blot: S100A6 Antibody (JF0976) [NBP2-67123] - Western blot analysis of S100A6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: mouse lung tissue lysate](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Western-Blot-NBP2-67123-img0011.jpg "Western Blot: S100A6 Antibody (JF0976) [NBP2-67123] - Western blot analysis of S100A6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A431 cell lysate Lane 2: mouse lung tissue lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IP |
| Clone | JF0976 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Recombinant full length protein of Human S100A6 aa 1-90 / 90. (SwissProt: P06703 Human; SwissProt: P14069 Mouse; SwissProt: P05964 Rat) |
| Localization | Nucleus envelope, Cytoplasm, Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | S100A6 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for S100A6 Antibody (NBP2-67123)Find related products by research area.
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S100A6: Playing Roles in Cancer, Apoptosis & Transcription Regulation S100A6 antibodies detect a small calcium binding protein with 2 EF-hand structures and belongs to the S100 family. Calcium binding induces a conformational change of the protein which in turn permits its interaction with several target proteins. It is... Read full blog post. |
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| Gene Symbol | S100A6 |
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunocytochemistry-Immunofluorescence-NBP2-67123-img0004.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in SH-SY-5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunohistochemistry-Paraffin-NBP2-67123-img0009.jpg "Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded mouse kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Flow-Cytometry-NBP2-67123-img0001.jpg "Flow Cytometry: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of SH-SY-5Y cells with S100 alpha 6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody")
![Western Blot: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse lung](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Western-Blot-NBP2-67123-img0010.jpg "Western Blot: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse lung")
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunocytochemistry-Immunofluorescence-NBP2-67123-img0002.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunocytochemistry-Immunofluorescence-NBP2-67123-img0003.jpg "Immunocytochemistry/Immunofluorescence: S100A6 Antibody (JF0976) [NBP2-67123] - Staining S100 alpha 6 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunohistochemistry-Paraffin-NBP2-67123-img0005.jpg "Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human colon cancer tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunohistochemistry-Paraffin-NBP2-67123-img0006.jpg "Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunohistochemistry-Paraffin-NBP2-67123-img0007.jpg "Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded human kidney tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S100A6-Antibody-JF0976-Immunohistochemistry-Paraffin-NBP2-67123-img0008.jpg "Immunohistochemistry-Paraffin: S100A6 Antibody (JF0976) [NBP2-67123] - Analysis of paraffin-embedded mouse brain tissue using anti-S100 alpha 6 antibody. Counter stained with hematoxylin.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")