![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Antibody (S100A1/1942).](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1942-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-59617-img0003.jpg "Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Antibody (S100A1/1942).")
| Reactivity | HuSpecies Glossary |
| Applications | IHC, MA |
| Clone | S100A1/1942 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Human Chromosome Location:1q21.3 Positive Control: A431 or HT-29 Cells. Brain or Melanoma 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59615). Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. |
| Immunogen | Recombinant human full-length S100A1 protein (Uniprot: P23297) |
| Localization | Cytoplasmic |
| Marker | Melanoma Marker |
| Specificity | The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the alpha-chain (i.e. in S-100A and S-100B) but not on the beta-chain of S-100 (i.e. in S-100B). This antibody can be used to localize S-100A in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, epithelial and myoepithelial cells of the breast, salivary and sweat glands. Neoplasms derived from these cells also express S-100 protein. Almost all malignant melanomas and cases of histiocytosis X are positive for S-100 protein. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | S100A1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed or Frozen): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
| Storage | Store at -20 to -80C. Avoid freeze-thaw cycles. |
| Buffer | 10 mM PBS |
| Preservative | No Preservative |
| Concentration | 1.0 mg/ml |
| Purity | Protein A or G purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for S100A1 Antibody (NBP2-59617)Find related products by research area.
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| Gene Symbol | S100A1 |
![Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942)](http://images.novusbio.com/fullsize/S100A1-Antibody-S100A1-1942-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-59617-img0002.jpg "Immunohistochemistry-Paraffin: S100A1 Antibody (S100A1/1942) - Azide and BSA Free [NBP2-59617] - Formalin-fixed, paraffin-embedded human Melanoma stained with S100A1 Mouse Monoclonal Antibody (S100A1/1942)")
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