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RTKN Antibody (OTI2E9) - Azide and BSA Free

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Western Blot: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RTKN.
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope retrieval ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Human bladder tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope ...read more
Immunohistochemistry: RTKN Antibody (OTI2E9) - Azide and BSA Free [NBP2-73950] - Staining of paraffin-embedded Human tonsil within the normal limits using anti-RTKN mouse monoclonal antibody. (Heat-induced epitope ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI2E9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

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RTKN Antibody (OTI2E9) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human RTKN(NP_149035) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
RTKN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for RTKN Antibody (OTI2E9) - Azide and BSA Free

  • B5
  • rhotekin
  • RTKN1

Background

The RTKN gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form b

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Bioinformatics

Gene Symbol RTKN