Novus Biologicals products are now on bio-techne.com

ROCK1 Antibody (154C1465) [Alexa Fluor® 647] - (active/cleaved)

Images

 
There are currently no images for cleaved ROCK1 Antibody (NB100-56596AF647).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Pm, RbSpecies Glossary
Applications WB, ICC/IF
Clone
154C1465
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

ROCK1 Antibody (154C1465) [Alexa Fluor® 647] - (active/cleaved) Summary

Immunogen
This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to amino acids 1114-1126 GNLPESRIEGWLS of human ROCK-1. This sequence is 100% conserved between human, chimpanzee, mouse, rat, dog, and rabbit. This immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1.
Specificity
This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
ROCK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human reactivity reported in scientific literature (PMID:32861242). Cross reacts with Chimpanzee.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ROCK1 Antibody (154C1465) [Alexa Fluor® 647] - (active/cleaved)

  • EC 2.7.11
  • EC 2.7.11.1
  • MGC131603
  • MGC43611
  • p160 ROCK-1
  • p160ROCK
  • p160-ROCK
  • PRO0435
  • Renal carcinoma antigen NY-REN-35
  • Rho kinase
  • rho-associated protein kinase 1
  • Rho-associated, coiled-coil containing protein kinase 1
  • Rho-associated, coiled-coil-containing protein kinase 1
  • ROCK1
  • ROK beta

Background

Rho-associated kinase I (ROCK1, ROK beta, p160ROCK) is a serine-threonine protein kinase and an effector of the small GTPase Rho. With N-terminus protein kinase domain and C-terminus Rho-binding domain/pleckstrin homology domain, ROCK1 can be activated by either RhoA or RhoB. In Rho specific ROCK1 activation, a Rho protein binds to the Rho-binding domain and induces a conformational change which opens the kinase domain for the phosphorylation of downstream effectors (1). Activated ROCK1 phosphorylates various signaling proteins, such as myosin light chain phosphatase, LIM kinases, and ezrin-radixin-moesin proteins. Caspase-3 also activates ROCK1; Caspase-3 cleaves ROCK1 at DETD1113/G sequence and removes its inhibitory c-terminal domain. This activation, independent of Rho activity, leads to apoptotic membrane blebbing (2). ROCK1 is involved in regulating actin cytoskeleton assembly, cell migration, centromere positing, smooth muscle contraction, and neurite outgrowth. Its involvement in tumor invasion, hypertension, and bronchial asthma makes ROCK1 an ideal target for drug development (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-58359
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
Supplier Logo
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Novus USA
Phone: 1-888-506-6887
Phone: 303-730-1950
Fax: 303-730-1966
Email: novus@novusbio.com
See More

Product PDFs

  SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our ROCK1 Antibody (154C1465) [Alexa Fluor® 647] - (active/cleaved) and receive a gift card or discount.

Bioinformatics

Gene Symbol ROCK1