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RNF8 Antibody

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Western Blot: RNF8 Antibody [NB100-1176] - Staining of HepG2 (A) and Jurkat (B) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Western Blot: RNF8 Antibody [NB100-1176] - Staining of HepG2 (A) and Jurkat (B) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: RNF8 Antibody [NB100-1176] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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RNF8 Antibody Summary

Immunogen
Peptide with sequence GEPGFFVTGDRAG-C corresponding to N-Terminus according to NP_003949.1, NP_898901.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
RNF8
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.5 - 2 ug/mL
Application Notes
WB: Approx. 55 kDa band observed in human placenta and lung lysates (predicted MW of 56 kDa band according to NP_003949).
Theoretical MW
56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
RNF8 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RNF8 Antibody

  • ActA Binding Protein 2
  • C3HC4-type zinc finger protein
  • EC 6.3.2
  • EC 6.3.2.-
  • FLJ12013
  • KIAA0646UBC13/UEV-interacting ring finger protein
  • ring finger protein (C3HC4 type) 8
  • ring finger protein 8E3 ubiquitin-protein ligase RNF8
  • RNF8

Background

The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Results suggest a novel function of RNF8 as a regulator of RXR alpha-mediated transcriptional activity through interaction between their respective N-terminal regions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

RNF8 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF8
Entrez
Uniprot