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RNF40 Antibody (SR1131)

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Western Blot: RNF40 Antibody (SR1131) [NBP3-22007] - Western blot analysis using NBP3-22007 in (1) HeLa cell lysate; (2) RAW264.7 cell lysate.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IP
Clone
SR1131
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RNF40 Antibody (SR1131) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthesized peptide derived from human RNF40 (Uniprot #: O75150)
Specificity
Detects endogenous levels of total RNF40
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
RNF40
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.4, 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for RNF40 Antibody (SR1131)

  • 95 kDa retinoblastoma-associated protein
  • BRE1-B
  • BRE1BMGC13051
  • EC 6.3.2,95 kDa retinoblastoma protein binding protein
  • EC 6.3.2.-
  • KIAA0661BRE1 E3 ubiquitin ligase homolog B
  • Rb-associated protein
  • RBP95E3 ubiquitin-protein ligase BRE1B
  • ring finger protein 40DKFZp686K191
  • STARING

Background

RNF40 is encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RNF40