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RIG-I Recombinant Protein Antigen

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Reactivity HuSpecies Glossary
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RIG-I Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human RIG-I.

Source: E. coli

Amino Acid Sequence: NREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVK

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.
Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
RIGI
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-55599.It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for RIG-I Recombinant Protein Antigen

  • DDX58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEAD box protein 58
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide
  • DKFZp686N19181
  • EC 3.6.4.13
  • FLJ13599
  • probable ATP-dependent RNA helicase DDX58
  • retinoic acid inducible gene I
  • Retinoic acid-inducible gene 1 protein
  • Retinoic acid-inducible gene I protein
  • RIG-1
  • RIGI
  • RIG-I
  • RIG-IDKFZp434J1111
  • RNA helicase RIG-I

Background

RIG-I, also known as DDX58, consists of a 106.6 kDa and a 101.4 kDa isoform and is involved in recognizing viruses and activating a response. Current research is being conducted with diseases and disorders such as hepatitis, rubella, influenza, rabies, hemorrhagic fever, lupus nephritis, and tick-borne encephalitis. The protein interacts in pathways such as NF-kappa signaling, cytosolic DNA-sensing, measles, influenza A, and mediated induction pathways with proteins such as CYLD, ZC3HAV1, DDX3X, MAVS, and WRNIP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol RIGI