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Rictor Antibody (7B3) [PE]

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Flow Cytometry: Rictor Antibody (7B3) [PE] [NBP1-51645PE] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with ...read more

Product Details

Summary
Reactivity Hu, Mu, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clone
7B3
Clonality
Monoclonal
Host
Mouse
Conjugate
PE

Order Details

Rictor Antibody (7B3) [PE] Summary

Immunogen
Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
RICTOR
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for Rictor Antibody (7B3) [PE]

  • AVO3 homolog
  • AVO3
  • hAVO3
  • KIAA1999DKFZp686B11164
  • mAVO3
  • MGC39830
  • PIA
  • Pianissimo
  • rapamycin-insensitive companion of mTOR
  • Rictor
  • RPTOR independent companion of MTOR, complex 2
  • TORC2-specific protein AVO3

Background

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rictor Antibody (NBP1-51645PE) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Rictor Antibody (NBP1-51645PE). (Showing 1 - 1 of 1 FAQ).

  1. I want to use antibody NBP1-51645PE to do Flow. Would you please provide the recommended dose/volume of this antibody?
    • For our Flow conjugates, we recommend using 0.5-1.0 ul of conjugated antibody per 10^6 (million) cells diluted into 100 ul of staining buffer as a starting point, see here.

Secondary Antibodies

 

Isotype Controls

Additional Rictor Products

Research Areas for Rictor Antibody (NBP1-51645PE)

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Blogs on Rictor.

mTOR - a central regulator of cell metabolism
The mammalian target of rapamycin (mTOR) signaling pathway allows cells to monitor environmental signals like nutrient availability and oxygen levels. mTOR is a phosphoinositide 3-kinase (PI3K)-related protein that assembles into large protein comp...  Read full blog post.

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Bioinformatics

Gene Symbol RICTOR