![Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Western-Blot-NBP1-51645-img0008.jpg "Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.")
| Reactivity | Hu, Mu, PmSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clone | 7B3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327] |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | RICTOR |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein in WB may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Theoretical MW | 192 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.03% Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Ammonium sulfate precipitation |
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reviewed by:
Verified Customer |
IHC-P | Human | 02/09/2022 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
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![Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) [NBP1-51645] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunocytochemistry-Immunofluorescence-NBP1-51645-img0006.jpg "Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) [NBP1-51645] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.")
![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Analysis of paraffin-embedded Human breast carcinoma using Rictor antibody. Antibody dilution 1:200. Image from verified customer review](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunohistochemistry-Paraffin-NBP1-51645-img0016.jpg "Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Analysis of paraffin-embedded Human breast carcinoma using Rictor antibody. Antibody dilution 1:200. Image from verified customer review")
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0015.jpg "Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Immunohistochemistry-Paraffin-NBP1-51645-img0007.jpg "Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.")
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0005.jpg "Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).")
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0012.jpg "Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.")
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0013.jpg "Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Rictor-Antibody-7B3-Flow-Cytometry-NBP1-51645-img0014.jpg "Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.")
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