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RICS Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: RICS Antibody [NBP1-92334] - Staining of human cell line HaCaT shows localization to nucleoplasm, nucleoli fibrillar center & the Golgi apparatus.
Immunohistochemistry-Paraffin: RICS Antibody [NBP1-92334] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry: RICS Antibody [NBP1-92334] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.
Immunohistochemistry-Paraffin: RICS Antibody [NBP1-92334] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: RICS Antibody [NBP1-92334] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: RICS Antibody [NBP1-92334] - Staining of human cerebellum shows moderate positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RICS Antibody [NBP1-92334] - Staining of human small intestine shows moderate to strong positivity in luminal membrane in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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RICS Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ERDPSVLYQYQPHGKRQSSVTVVSQYDNLEDYHSLPQHQRGVFGGGGMGTYVPPGFPHPQSRTYATALGQGAFLPAELSLQHPETQIHAE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGAP32
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
RICS Protein (NBP1-92334PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for RICS Antibody

  • brain-specific Rho GTP-ase-activating protein
  • Brain-specific Rho GTPase-activating protein
  • GAB-associated CDC42
  • GAB-associated Cdc42/Rac GTPase-activating protein
  • GC-GAPGTPase regulator interacting with TrkA
  • GRITp200RhoGAP
  • KIAA0712p250GAP
  • MGC1892
  • rac GTPase activating protein
  • Rho GTPase activating protein 32
  • rho GTPase-activating protein 32
  • Rho/Cdc42/Rac GTPase-activating protein RICS
  • RhoGAP involved in the beta-catenin-N-cadherin and NMDA receptor signaling
  • RhoGAP involved in the -catenin-N-cadherin and NMDA receptor signaling
  • Rho-type GTPase-activating protein 32
  • RICSPX-RICS

Background

RICS is a neuron-associated GTPase-activating protein that may regulate dendritic spine morphology and strength by modulating Rho GTPase (see RHOA; MIM 165390) activity (Okabe et al., 2003 (PubMed 12531901)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Bioinformatics

Gene Symbol ARHGAP32