Recombinant Rat TRANCE/RANK L/TNFSF11 Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

RANK L (receptor activator of NF-kappa B ligand), also called TRANCE (TNF-related activation-induced cytokines), OPGL (osteoprotegerin ligand), or ODF (osteoclast differentiation factor), is a 39-45 kDa type II transmembrane (TM) protein in the tumor necrosis factor family, designated TNFSF11 (1-5). RANK L, produced by osteoblasts and bone marrow stromal cells, is required for differentiation of osteoclasts and stimulates bone resorption (4, 6). It is also produced by activated T cells and augments dendritic cell stimulation; RANK L^-/- mice lack lymph nodes and have impaired thymocyte development (1-3, 6). The rat RANK Ligand cDNA encodes a type II transmembrane protein of 318 amino acids with a predicted cytoplasmic domain of 47 amino acids, a 21 amino acids transmembrane region, and an extracellular domain of 250 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Rat and human RANK Ligand and rat and mouse RANK Ligand share 83% and 95% amino acid identity respectively. RANK L can stimulate human osteoclast differentiation (4). Like most TNF family members, RANK L can form trimers (1). Soluble 31, 25 and 24 kDa forms of RANK L can be created by usage of alternate start sites at aa 74 or 146, or proteolytic cleavage by osteoblast- or stromal cell-derived ADAM10 (after aa 139) or MMP14 (aa 146), or bone metastatic prostate tumor-derived MT1-MMP (aa 146) (5, 7, 8). Both TM and soluble extracellular RANK L act by engaging RANK receptors and are antagonized by the decoy receptor, OPG (osteoprotegrin) (2, 5). In resting cells, the majority of RANK L is stored in secretory lysosomes (9). In mammary epithelia, RANK L is up-regulated by pregnancy hormones and is essential for the formation of a lactating mammary gland (10). In the brain, astrocyte RANK L mediates body temperature regulation (11). Pathologically, RANK L is thought to mediate post-menopausal osteoporosis, vascular calcification, progestin-induced breast cancer, cancer-induced bone disease, and osteopetrosis (in RANK L deficiencies) (12-16).

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 2 applications: Bioassay, Cell Culture.

Showing Publications 1 - 4 of 4.

Publications using 9366-TN	Applications	Species
B Meng, B Yang, Y Qu, Y Liu, D Wu, C Fu, Y He, X Chen, C Liu, X Kou, Y Cao Dual Role of Interleukin-20 in Different Stages of Osteoclast Differentiation and Its Osteoimmune Regulation during Alveolar Bone Remodeling International Journal of Molecular Sciences, 2023-02-14;24(4):. 2023-02-14 [PMID: 36835229] (Bioassay, Rat)	Bioassay	Rat
Y Liu, Y Ai, X Sun, B Meng, X Chen, D Wu, L Gan, B Yang, C Fu, Y Wu, Y Cao Interleukin-20 Acts as a Promotor of Osteoclastogenesis and Orthodontic Tooth Movement Stem Cells International, 2021-05-26;2021(0):5539962. 2021-05-26 [PMID: 34122555] (Bioassay, Rat)	Bioassay	Rat
Y Zeng, M Zhou, L Chen, H Fang, S Liu, C Zhou, J Sun, Z Wang Alendronate loaded graphene oxide functionalized collagen sponge for the dual effects of osteogenesis and anti-osteoclastogenesis in osteoporotic rats Bioact Mater, 2020-06-25;5(4):859-870. 2020-06-25 [PMID: 32637749] (Cell Culture, Rat)	Cell Culture	Rat
Q Tang, Z Hu, H Jin, G Zheng, X Yu, G Wu, H Liu, Z Zhu, H Xu, C Zhang, L Shen Microporous polysaccharide multilayer coated BCP composite scaffolds with immobilised calcitriol promote osteoporotic bone regeneration both in vitro and in vivo Theranostics, 2019-01-30;9(4):1125-1143. 2019-01-30 [PMID: 30867820] (Bioassay, Rat)	Bioassay	Rat

Array 9366-TN

• TRANCE/TNFSF11/RANK L Antibodies
• TRANCE/TNFSF11/RANK L Antibody Pair
• TRANCE/TNFSF11/RANK L Lysates
• TRANCE/TNFSF11/RANK L Proteins
• TRANCE/TNFSF11/RANK L Proteins and Enzymes
• TRANCE/TNFSF11/RANK L ELISAs