Recombinant Porcine IL-8/CXCL8 Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. This 8-9 kDa chemokine circulates as a monomer, homodimer, and heterodimer with CXCL4/PF4 (1, 2). Its oligomerization is modulated by interactions with matrix and cell surface glycosaminoglycans (GAGs) (3). Mature porcine CXCL8 shares 82%, 78%, and 65% amino acid (aa) sequence identiity with canine, feline, and human CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation of CXCL8 by multiple proteases generates a range of shorter forms (5). The bioactivity of CXCL8 is regulated by these truncations and also by CXCL8 citrullination (6). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). These receptors associate into functional homodimers and heterodimers with each other (7). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (8). It triggers the antimicrobial activation of neutrophils through CXCR1 (9). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (10). CXCL8 is up-regulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (11). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (12-15).

We have publications tested in 1 confirmed species: Porcine.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), ELISA Standard.

Showing Publications 1 - 6 of 6.

Publications using 535-IN	Applications	Species
MC Bonilla, L Fingerhut, A Alfonso-Ca, A Mergani, C Schwennen, M von Köckri, N de Buhr How Long Does a Neutrophil Live?-The Effect of 24 h Whole Blood Storage on Neutrophil Functions in Pigs Biomedicines, 2020-08-08;8(8):. 2020-08-08 [PMID: 32784433] (Bioassay, Porcine)	Bioassay	Porcine
SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines MethodsX, 2019-05-17;6(0):1218-1227. 2019-05-17 [PMID: 31193967] (ELISA Standard, Porcine)	ELISA Standard	Porcine
S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets Infect. Immun., 2017-03-23;85(4):. 2017-03-23 [PMID: 28167667] (ELISA (Standard), Porcine)	ELISA (Standard)	Porcine
Fishbourne E, Hutet E, Abrams C, Cariolet R, Le Potier M, Takamatsu H, Dixon L Increase in chemokines CXCL10 and CCL2 in blood from pigs infected with high compared to low virulence African swine fever virus isolates. Vet Res, 2013-10-01;44(0):87. 2013-10-01 [PMID: 24083897] (Bioassay, Porcine)	Bioassay	Porcine
Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology. Cytometry A, 2006-05-01;69(5):391-5. 2006-05-01 [PMID: 16604540] (ELISA (Standard))	ELISA (Standard)
Brix-Christensen V, Andersen SK, Andersen R, Mengel A, Dyhr T, Andersen NT, Schmitz O, Orskov H, Tonnesen E Acute hyperinsulinemia restrains endotoxin-induced systemic inflammatory response: an experimental study in a porcine model. Anesthesiology, 2004-04-01;100(4):861-70. 2004-04-01 [PMID: 15087621] (ELISA (Standard))	ELISA (Standard)

Array 535-IN

• CXCL8/IL-8 Antibodies
• CXCL8/IL-8 Antibody Pair
• CXCL8/IL-8 ELISA Kits
• CXCL8/IL-8 Kits
• CXCL8/IL-8 Lysate
• CXCL8/IL-8 Proteins
• CXCL8/IL-8 Proteins and Enzymes
• CXCL8/IL-8 ELISAs