Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 0.1-0.4 μg/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived mouse SCGF/CLEC11a protein Ala22-Phe328, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.
Applications/Dilutions
Dilutions
Bioactivity
Theoretical MW
35.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50 kDa, reducing conditions
Publications
Read Publication using 3729-SC/CF in the following applications:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse SCGF/CLEC11a Protein, CF
CLEC11a
CLECSF3
LSLCL
P47
SCGF
Background
Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature mouse SCGF contains an N‑terminal Glu‑rich region, a leucine zipper region, and a C‑terminal lectin‑like domain (1, 2). Human SCGF contains an RGD motif in its Glu‑rich region but rat SCGF does not (1). Mature mouse SCGF shares approximately 83% and 93% amino acid (aa) sequence identity with human and rat SCGF (1‑3). SCGF is produced by various hematopoietic cell lines and by CD34+ and CD34-CD33+ bone marrow cells as a 47 kDa sulfated and O‑glycosylated sialoprotein (2‑6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c‑Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU‑E) promoting activity in the absence of SCF/c‑kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL‑3, Flt‑3 Ligand, GM‑CSF, or G‑CSF to enhance the growth of granulocyte‑macrophage colonies (CFU‑GM) (4, 6). It also promotes (with Flt‑3 Ligand and VEGF) the maintenance and expansion of CD34+CD133+ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). In humans, circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (‑539C/T) is associated with resistance to malarial anemia (10, 11).
Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.
Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.
Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.
Hiraoka, A. et al. (1987) Cancer Res. 47:5025.
Ito, C. et al. (2003) Bone Marrow Transplant. 32:391.
Hiraoka, A. et al. (2001) Hematol. J. 2:307.
Da Riva, L. et al. (2011) J. Transl. Med. 9:158.
Gehling, U.M. et al. (2000) Blood 95:3106.
Loges, S. et al. (2004) Stem Cells Dev. 13:229.
Keller, C.C. et al. (2009) Infect. Immun. 77:3864.
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