Recombinant Mouse MFG-E8 Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C‑terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue‑protective role impairs anti‑tumor immunity and chemotherapy‑induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Porcine.

We have publications tested in 5 applications: Bioassay, Cell Culture, In Vivo, Reference Standard, Western Blot.

Showing Publications 1 - 10 of 37. Show All 37 Publications. Collapse Publications.

Publications using 2805-MF	Applications	Species
Ding, P;Liu, J;Meng, Y;Wang, H;Huang, Y;Su, G;Xia, C;Du, X;Dong, N;Cui, T;Zhang, J;Li, J; MFG-E8 facilitates heart repair through M1/M2 polarization after myocardial infarction by inhibiting CaMKII International immunopharmacology 2023-11-15 [PMID: 37977072] (In Vivo, Mouse)	In Vivo	Mouse
W Wu, J Wang, J Chen, J Lu, Y Lao, K Huang, J Lin MFG-E8 has guiding significance for the prognosis and treatment of sepsis Scientific Reports, 2022-12-03;12(1):20916. 2022-12-03 [PMID: 36463380] (In Vivo, Mouse)	In Vivo	Mouse
D Pan, W Wu, G Zuo, X Xie, H Li, X Ren, C Kong, W Zhou, Z Zhang, M Waterfall, S Chen Sphingosine 1-phosphate receptor 2 promotes erythrocyte clearance by vascular smooth muscle cells in intraplaque hemorrhage through MFG-E8 production Cellular Signalling, 2022-07-26;98(0):110419. 2022-07-26 [PMID: 35905868] (Bioassay, Mouse)	Bioassay	Mouse
R Geng, Y Lin, M Ji, Q Chang, Z Li, L Xu, W Zhang, J Lu MFG-E8 promotes tendon-bone healing by regualting macrophage efferocytosis and M2 polarization after anterior cruciate ligament reconstruction Journal of Orthopaedic Translation, 2022-05-11;34(0):11-21. 2022-05-11 [PMID: 35615640] (Cell Culture, Mouse)	Cell Culture	Mouse
B Brilland, P Laplante, P Thebault, K Geoffroy, MJ Brissette, M Latour, M Chassé, S Qi, MJ Hébert, H Cardinal, JF Cailhier MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy International Journal of Molecular Sciences, 2022-04-07;23(8):. 2022-04-07 [PMID: 35456911] (In Vivo, Mouse)	In Vivo	Mouse
M Ikemoto-Ue, H Zhou, T Kurosawa, Y Yoshimoto, M Toyoda, N Kanazawa, T Nakazawa, M Morita, K Tsuchida, A Uezumi Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation Aging Cell, 2021-12-24;0(0):e13536. 2021-12-24 [PMID: 34953020] (Reference Standard, Mouse)	Reference Standard	Mouse
Y Mao, SC Finnemann Acute RhoA/Rho Kinase Inhibition Is Sufficient to Restore Phagocytic Capacity to Retinal Pigment Epithelium Lacking the Engulfment Receptor MerTK Cells, 2021-07-29;10(8):. 2021-07-29 [PMID: 34440696] (Bioassay, Porcine)	Bioassay	Porcine
HY Chiang, PH Chu, SC Chen, TH Lee MFG-E8 Regulates Vascular Smooth Muscle Cell Migration Through Dose-Dependent Mediation of Actin Polymerization Journal of the American Heart Association, 2021-05-27;10(11):e020870. 2021-05-27 [PMID: 34041925] (In Vivo, Mouse)	In Vivo	Mouse
Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway Cell Death & Disease, 2021-05-25;12(6):533. 2021-05-25 [PMID: 34031369] (In Vivo, Mouse)	In Vivo	Mouse
HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice Cells, 2021-03-25;10(4):. 2021-03-25 [PMID: 33806041] (Bioassay, Mouse)	Bioassay	Mouse
NJ Esposito, F Mazzoni, JA Vargas, SC Finnemann Diurnal Photoreceptor Outer Segment Renewal in Mice Is Independent of Galectin-3 Investigative Ophthalmology & Visual Science, 2021-02-01;62(2):7. 2021-02-01 [PMID: 33538769] (Bioassay, Porcine)	Bioassay	Porcine
X Cai, Y Li, X Zheng, R Hu, Y Li, L Xiao, Z Wang Propofol suppresses microglial phagocytosis through the downregulation of MFG-E8 Journal of Neuroinflammation, 2021-01-09;18(1):18. 2021-01-09 [PMID: 33422097] (Bioassay, Mouse)	Bioassay	Mouse
HY Chiang, PH Chu, TH Lee MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells J. Biomed. Sci., 2019-08-30;26(1):61. 2019-08-30 [PMID: 31470852] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
J Li, X Xu, X Cai, Y Weng, Y Wang, Q Shen, X Shi Milk Fat Globule-Epidermal Growth Factor-Factor 8 Reverses Lipopolysaccharide-Induced Microglial Oxidative Stress Oxid Med Cell Longev, 2019-03-13;2019(0):2601394. 2019-03-13 [PMID: 31001372] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018-08-21;24(8):1939-1948.e4. 2018-08-21 [PMID: 30134156] (Western Blot, Mouse)	Western Blot	Mouse
AK Kumawat, C Yu, EA Mann, A Schridde, SC Finnemann, AM Mowat Expression and Characterisation of ?v?5 Integrin on Intestinal Macrophages Eur. J. Immunol., 2018-05-08;0(0):. 2018-05-08 [PMID: 29676784] (Bioassay, Mouse)	Bioassay	Mouse
MN Michalski, AL Seydel, EM Siismets, LE Zweifler, AJ Koh, BP Sinder, JI Aguirre, K Atabai, H Roca, LK McCauley Inflammatory bone loss associated with MFG-E8 deficiency is rescued by teriparatide FASEB J., 2018-02-22;0(0):fj201701238R. 2018-02-22 [PMID: 29475373] (Bioassay, Mouse)	Bioassay	Mouse
Y Wang, G Luo, J Chen, R Jiang, J Zhu, N Hu, W Huang, G Cheng, M Jia, B Su, N Zhang, T Cui Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions Sci Rep, 2017-02-14;7(0):42642. 2017-02-14 [PMID: 28195210] (Bioassay, Mouse)	Bioassay	Mouse
Mfge8 regulates enterocyte lipid storage by promoting enterocyte triglyceride hydrolase activity JCI Insight, 2016-11-03;1(18):e87418. 2016-11-03 [PMID: 27812539] (Bioassay, Mouse)	Bioassay	Mouse
A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016-09-23;6(0):33935. 2016-09-23 [PMID: 27659060] (Bioassay, Human)	Bioassay	Human
Marie-Joëlle Brissette Milk fat globule epidermal growth factor-8 limits tissue damage through inflammasome modulation during renal injury J Leukoc Biol, 2016-06-03;0(0):. 2016-06-03 [PMID: 27260955] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages Cancer Res, 2016-05-17;0(0):. 2016-05-17 [PMID: 27197197] (Bioassay, Mouse)	Bioassay	Mouse
Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes J Immunol, 2016-05-18;0(0):. 2016-05-18 [PMID: 27194784] (Bioassay, Mouse)	Bioassay	Mouse
Cheyuo C, Aziz M, Yang W, Jacob A, Zhou M, Wang P Milk fat globule-EGF factor VIII attenuates CNS injury by promoting neural stem cell proliferation and migration after cerebral ischemia. PLoS ONE, 2015-04-13;10(4):e0122833. 2015-04-13 [PMID: 25867181] (Bioassay, In Vivo, Mouse, Rat)	Bioassay, In Vivo	Mouse, Rat
Uchiyama A, Yamada K, Perera B, Ogino S, Yokoyama Y, Takeuchi Y, Ishikawa O, Motegi S Protective effect of MFG-E8 after cutaneous ischemia-reperfusion injury. J Invest Dermatol, 2014-12-10;135(4):1157-65. 2014-12-10 [PMID: 25493650] (Bioassay, Mouse)	Bioassay	Mouse
Soki F, Koh A, Jones J, Kim Y, Dai J, Keller E, Pienta K, Atabai K, Roca H, McCauley L Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis. J Biol Chem, 2014-07-08;289(35):24560-72. 2014-07-08 [PMID: 25006249] (Bioassay, Mouse)	Bioassay	Mouse
Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol, 2014-06-23;193(3):1383-91. 2014-06-23 [PMID: 24958900] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Neniskyte U, Vilalta A, Brown G Tumour necrosis factor alpha-induced neuronal loss is mediated by microglial phagocytosis. FEBS Lett, 2014-06-06;588(17):2952-6. 2014-06-06 [PMID: 24911209] (Bioassay, Mouse)	Bioassay	Mouse
Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S MFG-E8 regulates angiogenesis in cutaneous wound healing. Am J Pathol, 2014-05-15;184(7):1981-90. 2014-05-15 [PMID: 24838098] (In Vivo, Mouse)	In Vivo	Mouse
Wang X, Rodda L, Bannard O, Cyster J Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. J Immunol, 2014-04-16;192(10):4601-9. 2014-04-16 [PMID: 24740506] (Bioassay, Mouse)	Bioassay	Mouse
The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid beta toxicity. J Neuroinflammation, 2012-06-28;9(0):148. 2012-06-28 [PMID: 22742657] (Bioassay, Mouse)	Bioassay	Mouse
Aziz M, Matsuda A, Yang WL, Jacob A, Wang P Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2. J. Immunol., 2012-05-25;189(1):393-402. 2012-05-25 [PMID: 22634615] (In Vivo, Mouse)	In Vivo	Mouse
Brissette MJ, Lepage S, Lamonde AS, Sirois I, Groleau J, Laurin LP, Cailhier JF MFG-E8 released by apoptotic endothelial cells triggers anti-inflammatory macrophage reprogramming. PLoS ONE, 2012-04-30;7(4):e36368. 2012-04-30 [PMID: 22558449] (Bioassay, Mouse)	Bioassay	Mouse
Aziz M, Jacob A, Matsuda A, Wu R, Zhou M, Dong W, Yang WL, Wang P Pre-treatment of recombinant mouse MFG-E8 downregulates LPS-induced TNF-alpha production in macrophages via STAT3-mediated SOCS3 activation. PLoS ONE, 2011-11-15;6(11):e27685. 2011-11-15 [PMID: 22114683] (Bioassay, In Vivo, Mouse)	Bioassay, In Vivo	Mouse
Matsuda A, Wu R, Jacob A, Komura H, Zhou M, Wang Z, Aziz MM, Wang P Protective effect of milk fat globule-epidermal growth factor-factor VIII after renal ischemia-reperfusion injury in mice. Crit. Care Med., 2011-09-01;39(9):2039-47. 2011-09-01 [PMID: 21666453] (In Vivo, Mouse)	In Vivo	Mouse
Jinushi M, Sato M, Kanamoto A, Itoh A, Nagai S, Koyasu S, Dranoff G, Tahara H Milk fat globule epidermal growth factor-8 blockade triggers tumor destruction through coordinated cell-autonomous and immune-mediated mechanisms. J. Exp. Med., 2009-05-11;206(6):1317-26. 2009-05-11 [PMID: 19433619] (Bioassay, Mouse)	Bioassay	Mouse
Fuller AD, Van Eldik LJ MFG-E8 regulates microglial phagocytosis of apoptotic neurons. J Neuroimmune Pharmacol, 2008-08-01;3(4):246-56. 2008-08-01 [PMID: 18670887] (Bioassay, Mouse)	Bioassay	Mouse
Show All 37 Publications. Collapse Publications.

Array 2805-MF

• MFG-E8 Antibodies
• MFG-E8 Lysate
• MFG-E8 Protein
• MFG-E8 Proteins and Enzymes
• MFG-E8 ELISAs