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Recombinant Mouse Inhibin A Protein

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Recombinant Mouse Inhibin A (Catalog #8346-IN) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 5-30 ng/mL.
Recombinant Mouse Inhibin A (Catalog #8346-IN) neutralizes Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 5-30 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
8346-IN-010/CF
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Recombinant Mouse Inhibin A Protein Summary

Details of Functionality
Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED50 for this effect is 5-30 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Inhibin A protein
Ser234-Ile366 ( alpha chain) & Gly311-Ser426 ( beta chain)
Accession #
N-terminal Sequence
Ser234 ( alpha chain) & Gly311 ( beta chain)
Structure / Form
Disulfide-linked heterodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Inha
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
14.5 kDa ( alpha chain) & 13.0 kDa ( beta chain).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
12-14 kDa & 17-20 kDa, reducing conditions
Publications
Read Publications using
8346-IN in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in 4 mM HCl.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Inhibin A Protein

  • A-inhibin subunit
  • INHA
  • Inhibin A
  • inhibin alpha chain
  • inhibin, alpha

Background

Inhibin A is a member of the TGF-beta superfamily of proteins (1). Mature Inhibin A is a disulfide-linked dimer composed of alpha and beta subunits (1). Inhibin A shares a common alpha subunit with the closely related protein Inhibin B but has a unique beta subunit ( beta A) (2-4). The mature alpha subunit of mouse Inhibin A has a predicted molecular weight of 14.7 kDa and shares 80% and 97% amino acid (aa) sequence identity with the human and rat orthologs, respectively. The mature beta subunit of this mouse protein has a predicted molecular weight of 13 kDa and shares 93% and 100% aa sequence identity with the human and rat orthologs, respectively. Inhibin binds and antagonizes ActRIIA and ActRIIB in complex with the TGF-beta RIII and/or IGSF1 co-receptors and subsequently acts to suppress Activin-induced Follicle Stimulating Hormone (FSH) secretion (1, 5-7). Inhibins are produced by gonadal cells in both males and females (8). They are thought to be involved in the regulation of gametogenesis, and embryonic and fetal development (8, 9). Elevated concentrations of Inhibins are associated with pregnancy, preeclampsia, and ovarian cancer, and Inhibin A levels are typically measured during prenatal screening for Down’s syndrome (10-14).
  1. Phillips, D.J. and T.K. Woodruff (2004) Growth Factors 22:13.
  2. Ling, N. et al. (1985) Proc. Natl. Acad. Sci. USA 82:7217.
  3. Robertson, D.M. et al. (1985) Biochem. Biophys. Res. Commun. 126:220.
  4. Mason, A.J. et al. (1986) Biochem. Biophys. Res. Commun. 135:957.
  5. Lewis, K.A. et al. (2000) Nature 404:411.
  6. Martens, J.W. et al. (1997) Endocrinology 138:2928.
  7. Chapman, S.C. et al. (2002) Mol. Cell. Endocrinol. 196:79.
  8. de Kretser, D.M. and D.M. Robertson (1989) Biol. Reprod. 40:33.
  9. Knight, P.G. et al. (2012) Mol. Cell. Endocrinol. 359:53.
  10. Walentowicz, P. et al. (2014) PLoS One 9:e90575.
  11. Kondi-Pafiti, A. et al. (2013) Clin. Exp. Obstet. Gynecol. 40:109.
  12. Kuijper, E.A. et al. (2013) Reprod. Biomed. Online 27:33.
  13. Carty, D.M. et al. (2008) Trends Cardiovasc. Med. 18:186.
  14. Wald, N.J. et al. (1996) Prenat. Diagn. 16:143.

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Publications for Inhibin alpha (8346-IN)(2)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 2 applications: Cell Culture, Tissue Culture.


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(1)
Tissue Culture
(1)
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Immunohistochemistry-Paraffin: Inhibin alpha Antibody [NBP2-26096] - 60X image of Hu Testis stained with Inhibin alpha antibody at 1:50 shows questionable punctate or vesicular staining, both cytoplasmic and somewhat nuclear, in fluorescent green 488. Did not correspond completely with multiple other INHA antibodies tested. IHC staining with citrate pH 6 antigen retrieval; detected staining with a 488-conjugated anti-goat secondary antibody. IHC image submitted by a verified customer review.
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