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Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When 0.5 μg/mL of Recombinant Mouse IL‑5 R alpha /CD125 Fc Chimera is immobilized onto a goat anti-human IgG Fc antibody coated plate, the concentration of biotinylated recombinant mouse IL-5 that produces 50% of the optimal binding response is found to be approximately 4‑16 ng/mL
Source
Mouse myeloma cell line, NS0-derived mouse IL-5 R alpha/CD125 protein
Mouse IL-5 R alpha
(Asp18-His339)
Accession # P21183		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp18
Structure / Form

Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il5ra
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
63.2 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
75-85 kDa, 55 kDa and 35 kDa under reducing conditions
Publications
Read Publication using
553-MR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF

  • CD125 antigen
  • CD125
  • CDw125CD125
  • HSIL5R3
  • IL-5 R alpha
  • IL-5 receptor subunit alpha
  • IL5R alpha
  • IL-5R subunit alpha
  • IL5R
  • IL5RA
  • IL-5Ra
  • IL-5R-alpha
  • interleukin 5 receptor type 3
  • interleukin 5 receptor, alpha
  • interleukin-5 receptor alpha chain
  • interleukin-5 receptor alpha subunit
  • interleukin-5 receptor subunit alpha
  • MGC26560

Background

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1-3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha , respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common  beta  chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8-10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).
  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147.
  3. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925.
  4. Takaki, S. et al. (1990) EMBO J. 9:4367.
  5. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  6. Tavernier, J. et al. (1991) Cell 66:1175.
  7. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  8. Tavernier, J. et al. (2000) Blood 95:1600.
  9. Clutterbuck, E.J. et al. (1989) Blood 73:1504.
  10. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219.
  11. Denburg, J.A. et al. (1991) Blood 77:1462.
  12. Hasbold, J. et al. (2004) Nat. Immunol. 5:55.
  13. Gregory, B. et al. (2003) J. Immunol. 170:5359.
  14. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.

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Publications for IL-5R alpha/CD125 (553-MR)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol Il5ra
Uniprot