Recombinant Mouse IL-24 (NS0-expressed) Protein

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Interleukin 24 (IL-24), also known as FISP (IL-4 induced secreted protein) in mouse, and mda-7 (melanoma differentiation associated gene‑7) in humans, is a member of the IL-10 family of helical cytokines (1-3). The mouse IL-24 gene encodes for a 181 amino acid (aa) precursor that includes a 26 aa signal sequence and an 18 kDa (predicted), 155 aa mature segment (4). Unlike mature human IL-24, which contains three potential N-linked glycosylation sites, mouse mature IL-24 has only one. In human, both glycosylation and the presence of an intrachain disulfide bond have been found to be necessary for full activity (5). This requirement for glycosylation is unique among IL-10 family members. Presumably, the same structural modifications apply to mouse IL-24. Mouse IL-24 is a 27 kDa protein when secreted, and is active on human receptors (1, 3). Mature mouse IL-24 shares 69%, 84%, 69%, 68% and 61% aa sequence identity with human, rat, equine, canine and bovine IL-24, respectively. A 119 aa isoform termed FISP-sp diverges at aa 77. FISP-sp has been found mainly in the endoplasmic reticulum of Th2 cells. It can dimerize with intracellular IL-24, and antagonize the pro-apoptotic effects of IL-24 (6). Under physiological conditions, cytokine-stimulated monocytes/macrophages and Th2 cells produce most secreted IL-24; other sources include B cells, keratinocytes, NK cells, and differentiating melanoma cells (1-3, 7-9). Secreted IL-24 binds and signals through complexes of IL‑20 R alpha :IL-20 R beta , or IL-22 R:IL-20 R beta (10). The IL-20 R alpha :IL-20 R beta complex is also a receptor for IL-19 and IL-20, while IL-22 R:IL-20 R beta binds IL‑20 (2, 10).  Both receptors are widely expressed, but have not been found on hematopoietic cells (3). Even so, IL-24 induces type I proinflammatory cytokines in monocytes, and inhibits plasma cell differentiation in germinal center B cells (8, 11). The phenotype of mice transgenic for IL-24 is similar to that of IL-20 and IL-22 transgenic mice, indicating overlap in their activities, particularly in the skin (12). Secreted IL-24 is anti-angiogenic, binding receptors on endothelial cells and blocking their differentiation (13). Intratumoral injection of adenovirus coding for IL-24 causes tumor-specific apoptosis in humans, but it is not clear whether secreted IL-24 contributes to this effect (14).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: Bioassay, In Vivo.

Showing Publications 1 - 3 of 3.

Publications using 7807-ML	Applications	Species
A Ónody, A Veres-Szék, D Pap, R Rokonay, B Szebeni, E Sziksz, F Oswald, G Veres, Á Cseh, AJ Szabó, Á Vannay Interleukin-24 regulates mucosal remodeling in inflammatory bowel diseases Journal of Translational Medicine, 2021-06-02;19(1):237. 2021-06-02 [PMID: 34078403] (In Vivo, Mouse)	In Vivo	Mouse
AR Burmeister, MB Johnson, JJ Yaemmongko, I Marriott Murine astrocytes produce IL-24 and are susceptible to the immunosuppressive effects of this cytokine J Neuroinflammation, 2019-03-02;16(1):55. 2019-03-02 [PMID: 30825881] (Bioassay, Mouse)	Bioassay	Mouse
BX Ross, N Gao, X Cui, TJ Standiford, J Xu, FX Yu IL-24 Promotes Pseudomonas aeruginosa Keratitis in C57BL/6 Mouse Corneas J. Immunol, 2017-03-22;0(0):. 2017-03-22 [PMID: 28330899] (In Vivo, Mouse)	In Vivo	Mouse

Array 7807-ML

• IL-24 Antibodies
• IL-24 ELISA Kits
• IL-24 Lysate
• IL-24 ELISAs
• IL-24 Proteins and Enzymes