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Recombinant Mouse IL-18/IL-1F4 Protein

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Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) induces IFN gamma secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL.
Recombinant Mouse IL-18/IL-1F4 (Catalog # 9139-IL) induces IFN gamma secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL.
1 μg/lane of Recombinant Mouse IL-18 (Catalog # 9139-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing a single band at 18 kDa.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Carrier Free
9139-IL-050/CF
Carrier Free
9139-IL-010/CF
Carrier Free
9139-IL-500/CF
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Recombinant Mouse IL-18/IL-1F4 Protein Summary

Details of Functionality
Measured by its ability to induce mouse IFN-gamma secretion by activated mouse T cells. The ED50 for this effect is 0.06-0.36 ng/mL.
Source
E. coli-derived mouse IL-18/IL-1F4 protein
Asn36-Ser192
Accession #
N-terminal Sequence
Asn36 & Leu40
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 5
using
9139-IL in the following application:
Publications
Read Publications using
9139-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT with BSA as a carrier protein.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-18/IL-1F4 Protein

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature mouse IL-18 shares 63% and 91% amino acid sequence identity with mouse and rat IL-18, respectively (15).
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Okamura, H. et al. (1995) Nature 378:88.

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Publications for IL-18/IL-1F4 (9139-IL)(11)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(6)
Cell Culture
(1)
In Vivo
(4)
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Mouse
(11)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 9139-IL Applications Species
Tougaard, P;Pérez, MR;Steels, W;Huysentruyt, J;Verstraeten, B;Vetters, J;Divert, T;Gonçalves, A;Roelandt, R;Takahashi, N;Janssens, S;Buus, TB;Taghon, T;Leclercq, G;Vandenabeele, P; Type 1 immunity enables neonatal thymic ILC1 production Science advances 2024-01-19 [PMID: 38232171] (Bioassay, Mouse) Bioassay Mouse
LaMarche, NM;Hegde, S;Park, MD;Maier, BB;Troncoso, L;Le Berichel, J;Hamon, P;Belabed, M;Mattiuz, R;Hennequin, C;Chin, T;Reid, AM;Reyes-Torres, I;Nemeth, E;Zhang, R;Olson, OC;Doroshow, DB;Rohs, NC;Gomez, JE;Veluswamy, R;Hall, N;Venturini, N;Ginhoux, F;Liu, Z;Buckup, M;Figueiredo, I;Roudko, V;Miyake, K;Karasuyama, H;Gonzalez-Kozlova, E;Gnjatic, S;Passegué, E;Kim-Schulze, S;Brown, BD;Hirsch, FR;Kim, BS;Marron, TU;Merad, M; An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis Nature 2023-12-06 [PMID: 38057662] (Bioassay, Mouse) Bioassay Mouse
CM Schafer, S Martin-Alm, K Kurylowicz, N Dufton, L Osuna-Alma, ML Wu, CF Johnson, AV Shah, DO Haskard, A Buxton, E Willis, K Wheeler, S Turner, M Chlebicz, RP Scott, S Kovats, A Cleuren, GM Birdsey, AM Randi, CT Griffin Cytokine-Mediated Degradation of the Transcription Factor ERG Impacts the Pulmonary Vascular Response to Systemic Inflammatory Challenge bioRxiv : the preprint server for biology, 2023-02-11;0(0):. 2023-02-11 [PMID: 36798267] (Bioassay, Mouse) Bioassay Mouse
X Zhao, T Peng, X Cao, Y Hou, R Li, T Han, Z Fan, M Zhao, Y Chang, H Chen, C Li, X Huang In�vivo G-CSF treatment activates the GR-SOCS1 axis to suppress IFN-gamma secretion by natural killer cells Cell Reports, 2022-09-13;40(11):111342. 2022-09-13 [PMID: 36103837] (Bioassay, Mouse) Bioassay Mouse
HY Chiang, HH Lu, JN Sudhakar, YW Chen, NS Shih, YT Weng, JW Shui IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence Nature Communications, 2022-02-15;13(1):874. 2022-02-15 [PMID: 35169117] (Bioassay, Mouse) Bioassay Mouse
G Zhao, H Zhang, S Zhu, S Wang, K Zhu, Y Zhao, L Xu, P Zhang, J Xie, A Sun, Y Zou, J Ge Interleukin-18 accelerates cardiac inflammation and dysfunction during ischemia/reperfusion injury by transcriptional activation of CXCL16 Cellular Signalling, 2021-09-04;0(0):110141. 2021-09-04 [PMID: 34487815] (In Vivo, Mouse) In Vivo Mouse
PT Rudak, J Choi, KM Parkins, KL Summers, DN Jackson, PJ Foster, AI Skaro, K Leslie, VC McAlister, VK Kuchroo, W Inoue, O Lantz, SMM Haeryfar Chronic stress physically spares but functionally impairs innate-like invariant T�cells Cell Reports, 2021-04-13;35(2):108979. 2021-04-13 [PMID: 33852855] (In Vivo, Mouse) In Vivo Mouse
JA Moral, J Leung, LA Rojas, J Ruan, J Zhao, Z Sethna, A Ramnarain, B Gasmi, M Gururajan, D Redmond, G Askan, U Bhanot, E Elyada, Y Park, DA Tuveson, M Gönen, SD Leach, JD Wolchok, RP DeMatteo, T Merghoub, VP Balachandr ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity Nature, 2020-02-19;0(0):. 2020-02-19 [PMID: 32076273] (In Vivo, Mouse) In Vivo Mouse
C Ge, IR Monk, A Pizzolla, N Wang, JG Bedford, TP Stinear, GP Westall, LM Wakim Bystander Activation of Pulmonary Trm Cells Attenuates the Severity of Bacterial Pneumonia by Enhancing Neutrophil Recruitment Cell Rep, 2019-12-24;29(13):4236-4244.e3. 2019-12-24 [PMID: 31875535] (Cell Culture, Mouse) Cell Culture Mouse
IJ Jensen, CS Winborn, MG Fosdick, P Shao, MM Tremblay, Q Shan, SK Tripathy, CM Snyder, HH Xue, TS Griffith, JC Houtman, VP Badovinac Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections PLoS Pathog., 2018-10-31;14(10):e1007405. 2018-10-31 [PMID: 30379932] (Bioassay, Mouse) Bioassay Mouse
Show All 11 Publications.

Review for IL-18/IL-1F4 (9139-IL) (1) 51

Average Rating: 5
(Based on 1 review)

Reviews using 9139-IL:
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In vitro bioactivity in cell culture
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  5
reviewed by:
Sweta Ghosh
 In vitro bioactivity in cell culture 04/19/2024
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Summary

ApplicationIn vitro bioactivity in cell culture
LotDGEE0521041

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In vitro bioactivity in cell culture IL-18/IL-1F4 9139-IL
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 Sweta Ghosh
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Application: In vitro bioactivity in cell culture
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