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Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

 (10 Publications)
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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

Order Details

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Catalog# & Formulation Size Price
Standard Formulation
499-ML-025/CF
Carrier Free
499-ML-025/CF
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Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein Summary

Details of Functionality
Measured by its ability to inhibit the IL-12 dependent proliferation of mouse splenocytes activated with PHA and IL-2. The ED50 for this effect is 1-3 ng/mL in the presence of 0.3 ng/mL of mouse IL-12.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12/IL-23 p40 protein
Met23-Ser335
Accession #
N-terminal Sequence
Met23
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il12b
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
35.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
499-ML in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IL-12/IL-23 p40 Homodimer Protein

  • CLMF p40
  • CLMF
  • CLMF2
  • Cytotoxic lymphocyte maturation factor 40 kDa subunit
  • IL12 p40
  • IL-12 p40
  • IL-12 subunit p40
  • IL12B
  • IL-12B
  • IL-12BNK cell stimulatory factor chain 2
  • interleukin 12, p40
  • interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
  • interleukin-12 beta chain
  • interleukin-12 subunit beta
  • natural killer cell stimulatory factor, 40 kD subunit
  • NKSF
  • NKSF2
  • NKSF2IL12, subunit p40

Background

Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 has multiple effects on T cells and NK cells and is a key mediator in the development of Th1 cells.

IL-12 is a heterodimeric cytokine containing two disulfide-linked subunits, p35 and p40. Human and mouse IL-12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. Although mouse IL-12 is active on human or mouse IL-12 responsive cells, human IL-12 is not active on mouse cells.

The disulfide-linked mouse p40 homodimer can bind to IL-12 receptors and is an antagonist of IL-12 activities in vitro. The mouse p40 monomer is at least ten times less active than the homodimer as an IL-12 antagonist. At the present time, the existence and the physiological role of mouse p40 homodimer in vivo remains to be determined.

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Publications for IL-12/IL-23 p40 (499-ML)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(6)
ELISA (Standard)
(3)
In Vivo
(2)
Western Blot
(1)
All Applications
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Mouse
(6)
All Species
Showing Publications 1 - 10 of 10.
Publications using 499-ML Applications Species
S Mondal, M Kundu, M Jana, A Roy, SB Rangasamy, KK Modi, J Wallace, YA Albalawi, R Balabanov, K Pahan IL-12 p40 monomer is different from other IL-12 family members to selectively inhibit IL-12R&amp;beta1 internalization and suppress EAE Proc. Natl. Acad. Sci. U.S.A., 2020-08-19;117(35):21557-21567. 2020-08-19 [PMID: 32817415] (Bioassay, Mouse) Bioassay Mouse
E Marks, C Naudin, G Nolan, BJ Goggins, G Burns, SW Mateer, JK Latimore, K Minahan, M Plank, PS Foster, R Callister, M Veysey, MM Walker, NJ Talley, G Radford-Sm, S Keely Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation Mucosal Immunol, 2017-01-25;0(0):. 2017-01-25 [PMID: 28120851] (Western Blot) Western Blot
Anna Daria Young microglia restore amyloid plaque clearance of aged microglia EMBO J, 2016-12-21;0(0):. 2016-12-21 [PMID: 28007893] (Bioassay, Mouse) Bioassay Mouse
Abdi K, Singh N, Spooner E, Kessler B, Radaev S, Lantz L, Xiao T, Matzinger P, Sun P, Ploegh H Free IL-12p40 monomer is a polyfunctional adaptor for generating novel IL-12-like heterodimers extracellularly. J Immunol, 2014-05-12;192(12):6028-36. 2014-05-12 [PMID: 24821971] (Bioassay, Mouse) Bioassay Mouse
Jana M, Mondal S, Jana A, Pahan K Interleukin-12 (IL-12), but not IL-23, induces the expression of IL-7 in microglia and macrophages: implications for multiple sclerosis. Immunology, 2014-04-01;141(4):549-63. 2014-04-01 [PMID: 24224652] (Bioassay, Mouse) Bioassay Mouse
Brahmachari S, Pahan K Suppression of regulatory T cells by IL-12p40 homodimer via nitric oxide. J. Immunol., 2009-07-08;183(3):2045-58. 2009-07-08 [PMID: 19587012] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
An HJ, Jeong HJ, Um JY, Kim HM, Hong SH Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages. J Ethnopharmacol, 2006-03-10;106(3):418-24. 2006-03-10 [PMID: 16530364] (ELISA (Standard)) ELISA (Standard)
Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells. Blood, 2004-05-13;104(5):1404-10. 2004-05-13 [PMID: 15142882] (ELISA (Standard)) ELISA (Standard)
Huaux F, 107322, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D A profibrotic function of IL-12p40 in experimental pulmonary fibrosis. Interferon-gamma modulates articular chondrocyte and osteoblast metabolism through protein kinase R-independent and dependent mechanisms, 2002-09-01;169(5):2653-61. 2002-09-01 [PMID: 12193738] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse
Lukacs NW, Berlin A, Schols D, Skerlj RT, Bridger GJ AMD3100, a CxCR4 antagonist, attenuates allergic lung inflammation and airway hyperreactivity. Am. J. Pathol., 2002-04-01;160(4):1353-60. 2002-04-01 [PMID: 11943720] (ELISA (Standard)) ELISA (Standard)

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Bioinformatics

Gene Symbol Il12b
Uniprot