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Recombinant Mouse gp130 Fc Chimera Protein, CF

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Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG)inhibits IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in thepresence of Recombinant Human IL-6 R alpha (Catalog # 227‑SR). The ED50 forthis effect is 0.12‑1.2 ng/mL.
Recombinant Mouse gp130 Fc Chimera (Catalog # 468-MG)inhibits IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in thepresence of Recombinant Human IL-6 R alpha (Catalog # 227‑SR). The ED50 forthis effect ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse gp130 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR). The ED50 for this effect is 0.12-1.2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse gp130 protein
Mouse gp130
(Gln23-Glu617)
Accession # Q6PDI9		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln23 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Il6st
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
94 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115 kDa, reducing conditions
Publications
Read Publications using
468-MG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse gp130 Fc Chimera Protein, CF

  • CD130 antigen
  • CD130
  • CDw130
  • DKFZp564F053
  • gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form
  • gp130
  • IL-6 receptor subunit beta
  • IL-6R subunit beta
  • IL-6RB
  • IL-6R-beta
  • IL6ST
  • interleukin 6 signal transducer (gp130, oncostatin M receptor)
  • interleukin receptor beta chain
  • interleukin-6 receptor subunit beta
  • Interleukin-6 signal transducer
  • Membrane glycoprotein 130
  • membrane glycoprotein gp130
  • Oncostatin-M receptor subunit alpha

Background

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.

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Recombinant Mouse
gp130 Fc Chimera Prot ...
468-MG
Recombinant Mouse gp130 Fc Chimera Protein, CF
Species: Mu
Applications: Bioactivity

Publications for gp130/CD130 (468-MG)(10)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: Bioassay, Cell Culture, In Vivo.


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Bioassay
(2)
Cell Culture
(1)
In Vivo
(8)
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Mouse
(10)
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Showing Publications 1 - 10 of 10.
Publications using 468-MG Applications Species
Mukherjee, S;Ghosh, S;Bawali, S;Chatterjee, R;Saha, A;Sengupta, A;Keswani, T;Sarkar, S;Ghosh, P;Chakraborty, S;Khamaru, P;Bhattacharyya, A; Administration of soluble gp130Fc disrupts M-1 macrophage polarization, dendritic cell activation, MDSC expansion and Th-17 induction during experimental cerebral malaria International immunopharmacology 2023-07-24 [PMID: 37494839] (In Vivo, Mouse) In Vivo Mouse
Bermick, J;Watson, S;Lueschow, S;McElroy, SJ; The fetal response to maternal inflammation is dependent upon maternal IL-6 in a murine model Cytokine 2023-04-30 [PMID: 37130487] (In Vivo, Mouse) In Vivo Mouse
S Yoshizaki, T Tamaru, M Hara, K Kijima, M Tanaka, DJ Konno, Y Matsumoto, Y Nakashima, S Okada Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/&amp;beta1 integrin pathway Journal of Neuroinflammation, 2021-01-06;18(1):12. 2021-01-06 [PMID: 33407620] (Bioassay, Mouse) Bioassay Mouse
R Robinson, H Youngblood, H Iyer, J Bloom, TJ Lee, L Chang, Z Lukowski, W Zhi, A Sharma, S Sharma Diabetes Induced Alterations in Murine Vitreous Proteome Are Mitigated by IL-6 Trans-Signaling Inhibition Invest. Ophthalmol. Vis. Sci., 2020-09-01;61(11):2. 2020-09-01 [PMID: 32870245] (In Vivo, Mouse) In Vivo Mouse
R Robinson, M Srinivasan, A Shanmugam, A Ward, V Ganapathy, J Bloom, A Sharma, S Sharma Interleukin-6 trans-signaling inhibition prevents oxidative stress in a mouse model of early diabetic retinopathy Redox Biol, 2020-05-13;34(0):101574. 2020-05-13 [PMID: 32422539] (In Vivo, Mouse) In Vivo Mouse
G Trimova, K Yamagata, S Iwata, S Hirata, T Zhang, F Uemura, M Satoh, N Biln, S Nakayamada, WP Maksymowyc, Y Tanaka Tumour necrosis factor alpha promotes secretion of 14-3-3? by inducing necroptosis in macrophages Arthritis Res. Ther., 2020-02-12;22(1):24. 2020-02-12 [PMID: 32051018] (Cell Culture, Mouse) Cell Culture Mouse
A Dixit, J Bottek, AL Beerlage, J Schuettpel, S Thiebes, A Brenzel, C Garbers, S Rose-John, HW Mittrücker, A Squire, DR Engel Frontline Science: Proliferation of Ly6C(+) monocytes during urinary tract infections is regulated by IL-6 trans-signaling J. Leukoc. Biol., 2017-12-28;0(0):. 2017-12-28 [PMID: 28882904] (In Vivo, Mouse) In Vivo Mouse
H Tsukamoto, K Fujieda, M Hirayama, T Ikeda, A Yuno, K Matsumura, D Fukuma, K Araki, H Mizuta, H Nakayama, S Senju, Y Nishimura Soluble IL-6R expressed by myeloid cells reduces tumor-specific Th1 differentation and drives tumor progression Cancer Res, 2017-02-24;0(0):. 2017-02-24 [PMID: 28235765] (In Vivo, Mouse) In Vivo Mouse
Lee , Min Jeou, Lee , Jin-Ku, Choi , Ji Won, Lee , Chang-Se, Sim , Ji Hyun, Cho , Chung-Hy, Lee , Kwang-Ho, Cho , Ik-Hyun, Chung , Myung-He, Kim , Hang-Rae, Ye , Sang-Kyu Interleukin-6 induces S100A9 expression in colonic epithelial cells through STAT3 activation in experimental ulcerative colitis. PLoS ONE, 2012-09-04;7(9):e38801. 2012-09-04 [PMID: 22962574] (In Vivo, Mouse) In Vivo Mouse
Jones GW, McLoughlin RM, Hammond VJ, Parker CR, Williams JD, Malhotra R, Scheller J, Williams AS, Rose-John S, Topley N, Jones SA Loss of CD4+ T cell IL-6R expression during inflammation underlines a role for IL-6 trans signaling in the local maintenance of Th17 cells. J. Immunol., 2010-01-18;184(4):2130-9. 2010-01-18 [PMID: 20083667] (Bioassay, In Vivo, Mouse) Bioassay, In Vivo Mouse

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Bioinformatics

Gene Symbol Il6st
Uniprot