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Recombinant Mouse FGFR1 Fc Chimera Protein, CF

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Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR)inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferationof NR6R‑3T3mouse fibroblast cells. The ED50 for this effect is 15‑90 ...read more
2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog #10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR)conditions and visualized by Coomassie® blue staining, showing bands at 95-115 ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse FGFR1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL
Source
Mouse myeloma cell line, NS0-derived mouse FGFR1 protein
Mouse FGF R1
(Arg22-Ile376)
Accession # P16092-6
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Arg22
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95-115 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

  • basic fibroblast growth factor receptor 1
  • BFGFR
  • bFGF-R-1
  • CD331 antigen
  • CD331
  • CEK
  • EC 2.7.10
  • EC 2.7.10.1
  • FGF R1
  • FGFBR
  • FGFR1
  • FGFR-1
  • fibroblast growth factor receptor 1
  • FLGH3
  • FLJ99988
  • Flt-2
  • FLT2H4
  • Fms-like tyrosine kinase 2
  • fms-related tyrosine kinase 2
  • H2
  • H5
  • HBGFR
  • heparin-binding growth factor receptor
  • hydroxyaryl-protein kinase
  • KAL 2
  • KAL2
  • N-SAM
  • OGD
  • Proto-oncogene c-Fgr
  • soluble FGFR1 variant 1
  • soluble FGFR1 variant 2

Background

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).
  1. Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
  2. Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
  3. Cuiling, Li. et al. (2005) Dev. 132:4755.
  4. Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
  5. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
  6. Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
  7. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
  8. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.

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Publications for FGFR1 (10135-FR) (0)

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FAQs for FGFR1 (10135-FR). (Showing 1 - 2 of 2 FAQ).

  1. I would like to inquire whether you have samples of the following antibodies: NBP1-61338, NB600-1287, NBP1-19481, NBP1-19864; The datasheets for these antibodies show different molecular weights of the detected proteins so its no altogether clear to me which one detects the real protein. To sort this out, we would be using FGFR1-deficient cells and would very much appreciate if you could supply us with small aliquots of these antibodies.
    • FGFR1 has multiple isoforms and is subject to multiple PTM's. The molecular weight can vary greatly depending on experimental conditions and samples used. We fully guarantee all of our products for the listed applications and species. If you cannot get a product to work in an application or species stated on our datasheet, our technical service team will troubleshoot with you to get it to work. If a product still does not work after troubleshooting, you can receive a free of charge replacement product or a full refund. Due to our 100% guarantee, we do not offer free of charge samples of our products. If a smaller sample size is available, it will be listed on our product page.
  2. I am working in Multiple sclerosis group and mainly working with mice model and cell culture study. I need FGFR1 antibody against Mice for FACS analysis. If it is available can I get the sample to check with my cells? And any Oligodendrocyte markers against mice for FACS too.
    • We do have an antibody that has been tested in FLOW, NB600-1287, however it has not been tested in mouse. If you would like to test this antibody in mouse samples we have an Innovators Reward Program where we reward you for trying our antibody in species and applications that have not been previously tested.

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