Recombinant Mouse FGFR1 Fc Chimera Protein, CF Summary
Details of Functionality |
Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL
|
Source |
Mouse myeloma cell line, NS0-derived mouse FGFR1 protein Mouse FGF R1 (Arg22-Ile376) Accession # P16092-6
| IEGRMDP | Mouse IgG2A (Glu98-Lys330) | N-terminus | | C-terminus | |
|
Accession # |
|
N-terminal Sequence |
Arg22 |
Structure / Form |
Disulfide-linked homodimer
|
Protein/Peptide Type |
Recombinant Proteins |
Purity |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Endotoxin Note |
<0.10 EU per 1 μg of the protein by the LAL method. |
Applications/Dilutions
Dilutions |
|
Theoretical MW |
66 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
SDS-PAGE |
95-115 kDa, under reducing conditions |
Packaging, Storage & Formulations
Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
|
Buffer |
Lyophilized from a 0.2 μm filtered solution in PBS. |
Purity |
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Reconstitution Instructions |
Reconstitute at 500 μg/mL in PBS. |
Notes
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Recombinant Mouse FGFR1 Fc Chimera Protein, CF
Background
Fibroblast growth
factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as
cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists
of four members that differ in their ligand affinities and play essential roles
in the regulation of embryonic development, cell proliferation,
differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like
domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain
(1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and
rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth
factors and is involved in limb induction (2, 3). Alternative splice variants of
this gene have been reported. This product is the longest variant, isoform 1
(1, 4). FGF Rs play essential roles in the regulation of embryonic development,
cell proliferation, differentiation and migration (5). Upon FGF and heparan
sulfate binding, the FGF Rs form homodimers and the kinase domains are
activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).
- Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091.
- Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240.
- Cuiling, Li. et al. (2005) Dev. 132:4755.
- Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31.
- Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116.
- Pantoliano, M.W. et al. (1994) Biochemistry 33:10229.
- Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292.
- Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
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