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Recombinant Mouse CTLA-4 Fc Chimera Protein, CF

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Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL when stimulated with 1 μg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1).
Recombinant Mouse CTLA‑4 Fc Chimera (Catalog # 434-CT) inhibits IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.03-0.15 μg/mL when stimulated with 1 μg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse CTLA-4 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. Linsley, P.S. et al. (1991) J. Exp. Med. 174:561. The ED50 for this effect is 0.1-0.4 µg/mL when stimulated with 1 µg/mL Recombinant Human B7‑1/CD80 Fc Chimera (Catalog # 140-B1) in the presence of PHA.
Source
Mouse myeloma cell line, NS0-derived mouse CTLA-4 protein
Mouse CTLA-4
Ala37-Phe162
Accession #Q6GTR6		 IEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala37
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Ctla4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
41 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
55 kDa, reducing conditions
Publications
Read Publications using
434-CT/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CTLA-4 Fc Chimera Protein, CF

  • CD
  • CD152 antigen
  • CD152
  • CD152IDDM12
  • CD28
  • celiac disease 3
  • CELIAC3
  • CTLA4
  • CTLA-4
  • cytotoxic T-lymphocyte antigen 4
  • cytotoxic T-lymphocyte protein 4
  • Cytotoxic T-lymphocyte-associated antigen 4
  • cytotoxic T-lymphocyte-associated protein 4
  • cytotoxic T-lymphocyte-associated serine esterase-4
  • GRD4
  • GSE
  • ICOS
  • ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4

Background

CTLA-4 (cytotoxic T-lymphocyte-4, designated CD152), is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily (1, 2). Human or mouse CTLA-4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence, a 126 aa extracellular domain (ECD) with one Ig-like V-type domain, a 21 aa transmembrane (TM) sequence, and a 41 aa cytoplasmic sequence. It is found as a covalent homodimer of 41 ‑ 43 kDa (2) Within the ECD, mouse CTLA-4 shares 94% and 68 ‑ 71% aa sequence identity with rat and human/porcine/bovine/rabbit/feline/canine CTLA-4, respectively. A 174 aa form that lacks TM and cytoplasmic sequences (sCTLA-4) is possibly secreted (3 ‑ 5). Isoforms of 56 ‑ 79 aa that mainly contain parts of the cytoplasmic domain are reported. In mouse, an isoform lacking the Ig-like domain has ligand-independent inhibitory activity and is termed liCTLA-4 (6). CD28, which is structurally related to CTLA-4, is constitutively expressed on naïve T cells and promotes T cell activation when engaged by B7-2 on antigen-presenting cells (APC) within the immunological synapse (IS) (1, 7, 8). In contrast, CTLA-4 is recruited from intracellular vesicles to the IS beginning 1-2 days after T cell activation (2, 7, 8). It forms a linear lattice with B7-1 on APC, inducing negative regulatory signals and ending T cell activation (9). Abatacept, a therapeutic human CTLA-4-Ig fusion protein (trade name Orencia), competes with CD28 for B7-1 and B7-2 binding and has been used to antagonize T cell activation in autoimmune conditions and to enhance transplant survival (10). Mice deleted for CTLA-4 show no abnormalities until after birth, but then develop lethal autoimmune reactions due to continued T cell activation and poor control by regulatory T cells, which constitutively express CTLA-4 in wild-type mice and humans (11 ‑ 13).

  1. Harper, K. et al. (1991) J. Immunol. 147:1037.
  2. Teft, W.A. et al. (2006) Annu. Rev. Immunol. 24:65.
  3. Magistrelli, G. et al. (1999) Eur. J. Immunol. 29:3596.
  4. Tector, M. et al. (2009) BMC Immunol. 10:51.
  5. Oaks, M.K. and K.M. Hallett (2000) J. Immunol. 164:5015.
  6. Vijayakrishnan, L. et al. (2004) Immunity 20:563.
  7. Pentcheva-Hoang, T. et al. (2004) Immunity 21:401.
  8. Jansson, A. et al. (2005) J. Immunol 175:1575.
  9. Darlington, P.J. et al. (2005) J. Immunol. 175:996.
  10. Platt, A.M. et al. (2010) J. Immunol. 185:1558.
  11. Wing, K. et al. (2008) Science 322:271.
  12. Friedline, R.H. et al. (2009) J. Exp. Med. 206:421.
  13. Jain, N. et al. (2010) Proc. Natl. Acad. Sci. USA 107:1524.

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Recombinant Mouse
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434-CT/CF
Recombinant Mouse CTLA-4 Fc Chimera Protein, CF
Species: Mu
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Publications for CTLA-4 (434-CT/CF)(12)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Bioassay, ELISA (Standard), Flow Cytometry, In Vivo, Neutralization.


Filter By Application
Bioassay
(7)
ELISA (Standard)
(2)
Flow Cytometry
(1)
In Vivo
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(10)
All Species
Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using 434-CT/CF Applications Species
J Morimoto, M Matsumoto, R Miyazawa, H Yoshida, K Tsuneyama, M Matsumoto Aire suppresses CTLA-4 expression from the thymic stroma to control autoimmunity Cell Reports, 2022-02-15;38(7):110384. 2022-02-15 [PMID: 35172142] (Flow Cytometry, Mouse) Flow Cytometry Mouse
A Hanson, K Elpek, E Duong, L Shallberg, M Fan, C Johnson, M Wallace, GR Mabry, S Sazinsky, L Pepper, CJ Shu, S Sathyanara, S Zuerndorfe, T Simpson, M Gostissa, M Briskin, D Law, J Michaelson, CJ Harvey ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models PLoS ONE, 2020-09-24;15(9):e0239595. 2020-09-24 [PMID: 32970735] (Bioassay, Human) Bioassay Human
Sandin L, Eriksson F, Ellmark P, Loskog A, Totterman T, Mangsbo S Local CTLA4 blockade effectively restrains experimental pancreatic adenocarcinoma growth in vivo. Oncoimmunology, 2014-01-16;3(1):e27614. 2014-01-16 [PMID: 24701377] (ELISA (Standard), Mouse) ELISA (Standard) Mouse
Stojanovic A, Fiegler N, Brunner-Weinzierl M, Cerwenka A CTLA-4 is expressed by activated mouse NK cells and inhibits NK Cell IFN-gamma production in response to mature dendritic cells. J Immunol, 2014-03-31;192(9):4184-91. 2014-03-31 [PMID: 24688023] (Neutralization, Mouse) Neutralization Mouse
Peng , Yanmeng, Luo , Gaoxing, Zhou , Junyi, Wang , Xiaojuan, Hu , Jie, Cui , Yanyan, Li , Xian C, Tan , Jianglin, Yang , Sisi, Zhan , Rixing, Yang , Junjie, He , Weifeng, Wu , Jun CD86 is an activation receptor for NK cell cytotoxicity against tumor cells. PLoS ONE, 2013-12-11;8(12):e83913. 2013-12-11 [PMID: 24349559] (In Vivo, Mouse) In Vivo Mouse
Kowalczyk A, D'Souza C, Zhang L Cell-extrinsic CTLA4-mediated regulation of dendritic cell maturation depends on STAT3. Eur J Immunol, 2014-01-22;44(4):1143-55. 2014-01-22 [PMID: 24338929] (Bioassay, Mouse) Bioassay Mouse
Leitner , Judith, Rieger , Armin, Pickl , Winfried, Zlabinger , Gerhard, Grabmeier-Pfistershammer , Katharin, Steinberger , Peter TIM-3 does not act as a receptor for galectin-9. PLoS Pathog, 2013-03-21;9(3):e1003253. 2013-03-21 [PMID: 23555261] (Bioassay, Mouse) Bioassay Mouse
Khan A, Fu H, Tan L, Harper J, Beutelspacher S, Larkin D, Lombardi G, McClure M, George A Dendritic cell modification as a route to inhibiting corneal graft rejection by the indirect pathway of allorecognition. Eur J Immunol, 2013-01-18;43(3):734-46. 2013-01-18 [PMID: 23212959] (Bioassay, Mouse) Bioassay Mouse
Butte MJ, Keir ME, Phamduy TB, Sharpe AH, Freeman GJ Programmed death-1 ligand 1 interacts specifically with the B7-1 costimulatory molecule to inhibit T cell responses. Immunity, 2007-07-12;27(1):111-22. 2007-07-12 [PMID: 17629517] (Bioassay) Bioassay
Anderson KM, Czinn SJ, Redline RW, Blanchard TG Induction of CTLA-4-mediated anergy contributes to persistent colonization in the murine model of gastric Helicobacter pylori infection. J. Immunol., 2006-05-01;176(9):5306-13. 2006-05-01 [PMID: 16621997] (Bioassay, Mouse) Bioassay Mouse
Show All 12 Publications.

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Bioinformatics

Gene Symbol Ctla4
Uniprot