Home » CRISP-4 » CRISP-4 Proteins and Enzymes » Recombinant Mouse CRISP-4 Protein, CF
Novus Biologicals products are now on bio-techne.com

Recombinant Mouse CRISP-4 Protein, CF

 (1 Publication)
 See All Related
Submit a Review

Images

 
There are currently no images for CRISP-4 (5017-CR).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Recombinant Mouse CRISP-4 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of NTera‑2 human testicular embryonic carcinoma cells.

The ED50 for this effect is 0.3-1.5 μg/mL after a 1 hour incubation at
37 °C.

Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse CRISP-4 protein
Pro22-Lys250, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Leu23
Protein/Peptide Type
Recombinant Proteins
Gene
Crisp4
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
27.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
34-37 kDa, reducing conditions
Publications
Read Publication using
5017-CR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CRISP-4 Protein, CF

  • CRISP4
  • CRISP-4

Background

CRISP-4 is one of several CRISPs (cysteine-rich secretory proteins) that contain 16 invariant cysteine residues and show structural homolgy to snake, insect, and lizard venom proteins. These proteins consist of an N-terminal SCP/CAP domain, a hinge region, and a cysteine-rich CRISP domain. CRISPs are expressed in discrete but overlapping regions of the male reproductive tract and play a role in spermatozoa adhesion with Sertoli cells and oocytes, decapacitation, and the acrosomal reaction (1 - 3). CRISP-4 facilitates the progesterone-induced acrosomal reaction by inhibiting calcium conductance through the TRPM8 channel on sperm (4). Mouse CRISP-4 is secreted as a 32 kDa protein in the epididymis (primarily the caput region), vas deferens, and seminal vesicle (5, 6). It is also associated with spermatozoa from the testis and caudal epididymis as a 26 - 28 kDa molecule (6). Its expression is induced at the onset of puberty in an androgen-dependent fashion (5). Mouse and rat CRISP-1 are additionally expressed in salivary glands, while CRISP-4 is not (6). Expression patterns, genomic structure, and sequence conservation indicate that CRISP-4 in mouse and rat are the likely orthologs of human CRISP-1 (5, 6). Mature mouse CRISP-4 shares 90% and 64% aa sequence identity with rat CRISP-4 and human CRISP-1, respectively (5). Mature mouse CRISP-4 shares 38% - 43% aa sequence identity with mouse CRISP-1, -2, and -3.
  1. Koppers, A.J. et al. (2011) Asian J. Androl. 13:111.
  2. Gibbs, G.M. et al. (2008) Endocr. Rev. 29:865.
  3. Maeda, T. et al. (1998) Biochem. Biophys. Res. Commun. 248:140.
  4. Gibbs, G.M. et al. (2011) Proc. Natl. Acad. Sci. 108:7034.
  5. Jalkanen, J. et al. (2005) Biol. Reprod. 72:1268.
  6. Nolan, M.A. et al. (2006) Biol. Reprod. 74:984.

Customers Who Viewed This Item Also Viewed...

PSP94/MSMB
Antibody (OTI6C7)
NBP2-02450
Western Blot PSP94/MSMB Antibody (OTI6C7)
Immunocytochemistry/ Immunofluorescence PSP94/MSMB Antibody (OTI6C7)
Immunohistochemistry-Paraffin PSP94/MSMB Antibody (OTI6C7)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
CRISP-1 Antibody
(840017) [Unconjugate ...
MAB4675
Western Blot CRISP-1 Antibody (840017) [Unconjugated]
Immunohistochemistry CRISP-1 Antibody (840017) [Unconjugated]
Neutralization CRISP-1 Antibody (840017) [Unconjugated]
(1 Publication)
Species: Mu
Applications: IHC, Neut, WB
CRISP-2 Antibody
NBP1-52012
Immunohistochemistry-Paraffin CRISP-2 Antibody
Western Blot CRISP-2 Antibody
(1 Publication)
Species: Ca, Hu, Po
Applications: IHC, IHC-P, PEP-ELISA, WB
Kallikrein 3/PSA
[HRP]
DKK300
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
N/A Kallikrein 3/PSA [HRP]
(17 Publications)
Species: Hu
Applications: ELISA
Recombinant Human
TMPRSS13 GST (N-Term) ...
H00084000-Q01
SDS-PAGE Recombinant Human TMPRSS13 GST (N-Term) Protein
Species: Hu
Applications: ELISA, AP, PA, PAGE, WB
MSP/MST1
[Unconjugated]
352-MS
MSP/MST1 [Unconjugated]
(9 Publications)
Species: Hu
Applications: BA, BA
Invadolysin
Antibody
NBP1-92025
Western Blot Invadolysin Antibody
Western Blot Invadolysin Antibody
Immunohistochemistry-Paraffin Invadolysin Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) - BSA Free
(14 Publications)
Species: Ca, Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
Protein S/PROS1
Antibody [Unconjugate
AF4036
Western Blot Protein S/PROS1 Antibody [Unconjugated]
Simple Western Protein S/PROS1 Antibody [Unconjugated]
(7 Publications)
Species: Hu
Applications: Simple Western, WB
PSAT1 Antibody
NBP1-32920
Western Blot PSAT1 Antibody
Immunocytochemistry/ Immunofluorescence PSAT1 Antibody
Immunohistochemistry-Paraffin PSAT1 Antibody
(12 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, KD, WB

Puromycin-sensitive am ...
NBP2-13669
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Immunohistochemistry-Paraffin Puromycin-sensitive aminopeptidase/NPEPPS Antibody
Species: Hu
Applications: ICC/IF, IHC, IHC-P
PLAG1 Antibody
(3B7)
H00005324-M02
Immunohistochemistry PLAG1 Antibody (3B7)
Immunohistochemistry PLAG1 Antibody (3B7)
ELISA PLAG1 Antibody (3B7)
(20 Publications)
Species: Hu, Mu
Applications: ELISA, IHC, IHC-P, WB
POU2F2 Antibody
(OCT2/2137)
NBP2-75734
Immunohistochemistry-Paraffin POU2F2 Antibody (OCT2/2137)
Immunohistochemistry-Paraffin POU2F2 Antibody (OCT2/2137)
Western Blot POU2F2 Antibody (OCT2/2137)
Species: Hu
Applications: ELISA, IHC, IHC-P, PA, WB
Peroxiredoxin 2
Antibody (4E10-2D2)
H00007001-M01
ELISA Peroxiredoxin 2 Antibody (4E10-2D2)
Western Blot Peroxiredoxin 2 Antibody (4E10-2D2)
Western Blot Peroxiredoxin 2 Antibody (4E10-2D2)
(1 Review)
(6 Publications)
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
alpha
1B-Glycoprotein Antib ...
AF8537
Western Blot alpha 1B-Glycoprotein Antibody [Unconjugated]
Immunocytochemistry alpha 1B-Glycoprotein Antibody [Unconjugated]
Simple Western alpha 1B-Glycoprotein Antibody [Unconjugated]
(1 Publication)
Species: Mu, Rt
Applications: ICC, Simple Western, WB
PARN Antibody
(OTI4D12)
NBP2-46321
Immunohistochemistry PARN Antibody (OTI4D12)
Immunohistochemistry PARN Antibody (OTI4D12)
Immunohistochemistry PARN Antibody (OTI4D12)
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
Clusterin/APOJ
Antibody [Unconjugate
AF2747
Immunohistochemistry Clusterin/APOJ Antibody [Unconjugated]
Immunohistochemistry Clusterin/APOJ Antibody [Unconjugated]
Western Blot Clusterin/APOJ Antibody [Unconjugated]
(13 Publications)
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, IP, WB
DAN/NBL1 Antibody
[Unconjugated]
AF755
DAN/NBL1 Antibody [Unconjugated]
(1 Publication)
Species: Mu
Applications: Block, WB

Publications for CRISP-4 (5017-CR)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ThT Fluorescence.


Filter By Application
ThT Fluorescence
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for CRISP-4 (5017-CR) (0)

There are no reviews for CRISP-4 (5017-CR). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CRISP-4 (5017-CR) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CRISP-4 Products

Blogs on CRISP-4

There are no specific blogs for CRISP-4, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  PDF SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse CRISP-4 Protein, CF and receive a gift card or discount.


Bioinformatics

Gene Symbol Crisp4
Uniprot