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Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

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When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED50 of 15-90 ng/mL.
When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 μg/mL (100 μL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED50 of 15-90 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF Summary

Additional Information
Biotinylated
Details of Functionality
The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF-C (Catalog # 9199-VC) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag (Catalog # AVI10384) binds with an ED50 of 15-90 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human VEGFR3/Flt-4 protein
Human VEGFR3/Flt-4
(Tyr25-Glu775)
Accession # P35916.3		HHHHHHAvi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Tyr25
Structure / Form
Monomer, biotinylated via Avi-tag
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
  • Bioactivity2
Theoretical MW
87 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
115-140 kDa, 72-89 kDa and 60-70 kDa

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt‑1) and VEGFR3 (Flt‑4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 is composed of a 751 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 482 aa residue cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt‑4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

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Recombinant Human
VEGFR3/Flt-4 His-tag ...
AVI10384
Recombinant Human VEGFR3/Flt-4 His-tag Avi-tag Protein, CF
Species: Hu
Applications: Bioactivity

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