Recombinant Human MFG-E8 Protein, CF

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66-75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Human MGF-E8 contains one N-terminal EGF-like domain and two C‑terminal F5/8-type discoidin-like domains (2). It shares 63% and 61% aa sequence identity with comparable regions of mouse and rat MFG-E8, respectively. Shorter isoforms of human MFG-E8 may have N-terminal deletions (beginning near the end of the first discoidin-like domain), internal deletions (lacking either the EGF-like domain or the central region of the second discoidin-like domain), or C‑terminal deletions (truncated within the second discoidin-like domain) (3). A 50 aa internal proteolytic fragment of human MFG-E8 (known as Medin) is a major component of aortic medial amyloid deposits (4). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (5, 6). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (7). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (8, 9) and of apoptotic B cells during germinal center reactions (10, 11). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (12, 13). Its tissue-protective role impairs anti‑tumor immunity and chemotherapy-induced apoptosis (14). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (15).

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Capture, In Vivo, Surface Plasmon Resonance.

Showing Publications 1 - 10 of 24. Show All 24 Publications. Collapse Publications.

Publications using 2767-MF	Applications	Species
Brink, SCA;Koolen, L;Klaver, CCW;Bakker, RA;den Hollander, AI;Almedawar, S; Non-canonical roles of CFH in retinal pigment epithelial cells revealed by dysfunctional rare CFH variants Stem cell reports 2024-12-17 [PMID: 39753135] (Bioassay, Human)	Bioassay	Human
Urban, L;Novák, Š;?oma, M;Dvo?ánková, B;Lacina, L;Šáchová, J;Hradilová, M;Svato?ová, P;Kolá?, M;Strnad, H;B?ezinová, J;Smetana, K;Gál, P;Szabo, P; Unravelling heterogeneous effects of cancer?associated fibroblasts on poor prognosis markers in breast cancer EM?G3 cell line: In vitro?targeted treatment (anti?IL-6, anti?VEGF-A, anti?MFGE8) based on transcriptomic profiling Oncology reports 2024-01-01 [PMID: 37975220] (Bioassay, Human)	Bioassay	Human
Tabasum, S;Thapa, D;Giobbie-Hurder, A;Weirather, JL;Campisi, M;Schol, PJ;Li, X;Li, J;Yoon, CH;Manos, MP;Barbie, DA;Hodi, FS; EDIL3 as an angiogenic target of immune exclusion following checkpoint blockade Cancer immunology research 2023-09-20 [PMID: 37728484] (ELISA Capture, Human)	ELISA Capture	Human
Chaung, W;Zhang, F;Ma, G;Yen, H;Jacob, A;Brenner, M;Wang, P; Development of human cell-expressed tag-free rhMFG-E8 as a radiation mitigator and a therapeutic for acute kidney injury Research square 2023-05-15 [PMID: 37293000] (Bioassay, Human)	Bioassay	Human
Y Cui, L Luo, Z Zeng, X Liu, T Li, X He, Y Ma, W Meng, H Zeng, Y Long, D Zong, Y Chen MFG-E8 stabilized by deubiquitinase USP14 suppresses cigarette smoke-induced ferroptosis in bronchial epithelial cells Cell Death & Disease, 2023-01-03;14(1):2. 2023-01-03 [PMID: 36596780] (Bioassay, Human)	Bioassay	Human
P Marazuela, M Solé, A Bonaterra-, J Pizarro, J Camacho, E Martínez-S, HB Kuiperij, MM Verbeek, AM de Kort, FHBM Schreuder, CJM Klijn, L Castillo-R, O Pancorbo, D Rodríguez-, F Pujadas, P Delgado, M Hernández- MFG-E8 (LACTADHERIN): a novel marker associated with cerebral amyloid angiopathy Acta neuropathologica communications, 2021-09-16;9(1):154. 2021-09-16 [PMID: 34530925] (Bioassay, Human)	Bioassay	Human
K Han, K Singh, MJ Rodman, S Hassanzade, Y Baumer, RD Huffstutle, J Chen, J Candia, F Cheung, KER Stagliano, M Pirooznia, TM Powell-Wil, MN Sack Identification and Validation of Nutrient State-Dependent Serum Protein Mediators of Human CD4+ T Cell Responsiveness Nutrients, 2021-04-28;13(5):. 2021-04-28 [PMID: 33924911] (Bioassay, Human)	Bioassay	Human
Z Shi, Q Wang, Y Zhang, D Jiang Extracellular vesicles produced by bone marrow mesenchymal stem cells attenuate renal fibrosis, in part by inhibiting the RhoA/ROCK pathway, in a UUO rat model Stem Cell Res Ther, 2020-06-26;11(1):253. 2020-06-26 [PMID: 32586368] (Bioassay, In Vivo, Human, Rat)	Bioassay, In Vivo	Human, Rat
Y Kanzaki, H Fujita, K Sato, M Hosokawa, H Matsumae, F Shiraga, Y Morizane, H Ohuchi KCNJ13 Gene Deletion Impairs Cell Alignment and Phagocytosis in Retinal Pigment Epithelium Derived from Human-Induced Pluripotent Stem Cells Invest. Ophthalmol. Vis. Sci., 2020-05-11;61(5):38. 2020-05-11 [PMID: 32437550] (Bioassay, Human)	Bioassay	Human
HY Chiang, PH Chu, TH Lee MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells J. Biomed. Sci., 2019-08-30;26(1):61. 2019-08-30 [PMID: 31470852] (Bioassay, Human)	Bioassay	Human
Z Pan, EK Dumas, C Lawrence, L Pate, S Longobardi, X Wang, JA James, S Kovats, AD Farris Bacillus anthracis Edema Toxin Inhibits Efferocytosis in Human Macrophages and Alters Efferocytic Receptor Signaling Int J Mol Sci, 2019-03-07;20(5):. 2019-03-07 [PMID: 30866434] (Bioassay, Human)	Bioassay	Human
YY Gao, ZH Zhang, Z Zhuang, Y Lu, LY Wu, ZN Ye, XS Zhang, CL Chen, W Li, CH Hang Recombinant milk fat globule-EGF factor-8 reduces apoptosis via integrin ?3/FAK/PI3K/AKT signaling pathway in rats after traumatic brain injury Cell Death Dis, 2018-08-28;9(9):845. 2018-08-28 [PMID: 30154436] (In Vivo, Rat)	In Vivo	Rat
MA Calton, MO Beaulieu, G Benchorin, D Vollrath Method for measuring extracellular flux from intact polarized epithelial monolayers Mol. Vis., 2018-06-25;24(0):425-433. 2018-06-25 [PMID: 30034209] (Bioassay, Human)	Bioassay	Human
Y Nakashima, C Miyagi-Shi, H Noguchi, T Omasa Atorvastatin Inhibits the HIF1?-PPAR Axis, Which Is Essential for Maintaining the Function of Human Induced Pluripotent Stem Cells Mol. Ther., 2018-06-19;0(0):. 2018-06-19 [PMID: 29929789] (Bioassay, Human)	Bioassay	Human
G Inana, C Murat, W An, X Yao, IR Harris, J Cao RPE phagocytic function declines in age-related macular degeneration and is rescued by human umbilical tissue derived cells J Transl Med, 2018-03-13;16(1):63. 2018-03-13 [PMID: 29534722] (Bioassay, Human)	Bioassay	Human
C Müller, C Charniga, S Temple, SC Finnemann Quantified F-Actin Morphology Is Predictive of Phagocytic Capacity of Stem Cell-Derived Retinal Pigment Epithelium Stem Cell Reports, 2018-02-15;0(0):. 2018-02-15 [PMID: 29456184] (Bioassay, Human)	Bioassay	Human
Q Zhao, L Xu, X Sun, K Zhang, H Shen, Y Tian, F Sun, Y Li MFG-E8 overexpression promotes colorectal cancer progression via AKT/MMPs signalling Tumour Biol., 2017-06-01;39(6):1010428317707. 2017-06-01 [PMID: 28653875] (Bioassay, Human)	Bioassay	Human
A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016-09-23;6(0):33935. 2016-09-23 [PMID: 27659060] (Bioassay, Human)	Bioassay	Human
Heo K, Cushman H, Akaike M, Woo C, Wang X, Qiu X, Fujiwara K, Abe J ERK5 activation in macrophages promotes efferocytosis and inhibits atherosclerosis. Circulation, 2014-04-28;130(2):180-91. 2014-04-28 [PMID: 25001623] (Bioassay, Mouse)	Bioassay	Mouse
Abe T, Shin J, Hosur K, Udey M, Chavakis T, Hajishengallis G Regulation of osteoclast homeostasis and inflammatory bone loss by MFG-E8. J Immunol, 2014-06-23;193(3):1383-91. 2014-06-23 [PMID: 24958900] (Bioassay, Human)	Bioassay	Human
Pliyev B, Kalintseva M, Abdulaeva S, Yarygin K, Savchenko V Neutrophil microparticles modulate cytokine production by natural killer cells. Cytokine, 2013-12-16;65(2):126-9. 2013-12-16 [PMID: 24355430] (Bioassay, Human)	Bioassay	Human
Lankry D, Rovis T, Jonjic S, Mandelboim O The interaction between CD300a and phosphatidylserine inhibits tumor cell killing by NK cells. Eur J Immunol, 2013-06-21;43(8):2151-61. 2013-06-21 [PMID: 23640773] (Cell Culture, Human)	Cell Culture	Human
Lin YP, Hsu ME, Chiou YY Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model. Proteomics, 2010-07-01;10(13):2429-43. 2010-07-01 [PMID: 20405472] (ELISA (Standard))	ELISA (Standard)
Toth B, Garabuczi E, Sarang Z, Vereb G, Vamosi G, Aeschlimann D, Blasko B, Becsi B, Erdodi F, Lacy-Hulbert A, Zhang A, Falasca L, Birge RB, Balajthy Z, Melino G, Fésüs L, Szondy Z Transglutaminase 2 is needed for the formation of an efficient phagocyte portal in macrophages engulfing apoptotic cells. J. Immunol., 2009-02-15;182(4):2084-92. 2009-02-15 [PMID: 19201861] (Surface Plasmon Resonance, Human)	Surface Plasmon Resonance	Human
Show All 24 Publications. Collapse Publications.

Array 2767-MF

• MFG-E8 Antibodies
• MFG-E8 Lysate
• MFG-E8 Protein
• MFG-E8 Proteins and Enzymes
• MFG-E8 ELISAs