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Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

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Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human IGF1R/C-His Protein (Catalog # 305-GR) has a molecular weight (MW) of 296.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a heterotetramer.  MW may differ from predicted MW due to ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

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Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF Summary

Additional Information
Analyzed by SEC-MALS
Details of Functionality
Measured by its binding ability in a functional ELISA. When Recombinant Human IGF‑I R/IGF1R is present at 0.5 μg/mL, the concentration of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1) that produces 50% of the optimal binding response is found to be approximately 2‑10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IGF-I R/IGF1R protein
Met1-Asn932 with a C-terminal 10-His tag
Accession #
N-terminal Sequence
Glu31 and Asp741
Protein/Peptide Type
Recombinant Proteins
Gene
IGF1R
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Binding Activity
Theoretical MW
104 kDa (single chain), 81 kDa ( alpha subunit) and 23 kDa ( beta subunit).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
150 kDa, 120 kDa and 48 kDa, reducing conditions
Publications
Read Publications using
305-GR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl, EDTA and Tween®-20 with Trehalose.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-I R/IGF1R (His-tagged) Protein, CF

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC 2.7.10.1
  • IGF1R
  • IGF-1R
  • IGF-I R
  • IGF-I receptor
  • IGFIR
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2

Background

IGF-I receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta  subunits. Both the alpha  and beta  subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I receptor is extracellular while the beta  subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R.

  1. Rechler, M.M. and S.P. Nissley (1990) Insulin-Like Growth Factors, in Peptide Growth Factors and Their Receptors I, M.B. Sporn and A.B. Roberts, editors, Springer-Verlag, New York, New York, p. 263.

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Publications for IGF-I R/IGF1R (305-GR)(2)

We have publications tested in 2 confirmed species: Human, Yeast.

We have publications tested in 2 applications: Bioassay, IHC-P.


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(1)
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(1)
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Bioinformatics

Gene Symbol IGF1R
Uniprot