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Recombinant Human IFN-gamma R2 His-tag Protein, CF

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Recombinant Human IFN-gamma R2 His-tag (Catalog # 11576-GR) binds Recombinant Human IFN-gamma (285-IF) in the presence of Recombinant Human IFN-gamma R1/CD119 (673-IR/CF) with an ED50 of 0.200‑3.00 μg/mL.
2 μg/lane of Recombinant Human IFN-gamma R2 His-tag Protein (Catalog # 11576-GR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

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Recombinant Human IFN-gamma R2 His-tag Protein, CF Summary

Accession #
N-terminal Sequence
Ala22
Protein/Peptide Type
Recombinant Proteins

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
38-42 kDa, under reducing conditions

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-gamma R2 His-tag Protein, CF

  • AF-1
  • AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1
  • IFGR2
  • IFN-gamma R2
  • IFN-gamma receptor 2
  • IFNgammaR2
  • IFN-gamma-R2
  • IFNGR2
  • IFN-gR2
  • IFNGT1
  • interferon gamma receptor 2 (interferon gamma transducer 1)
  • interferon gamma receptor 2
  • interferon gamma receptor accessory factor-1
  • interferon gamma receptor beta chain
  • Interferon gamma transducer 1

Background

IFN‑ gamma  R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma  RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma , the complex contains two each of IFN‑ gamma  R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma  R1 recruits Jak2 to IFN‑ gamma  R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3).  Within the ECD, human IFN‑ gamma  R2 shares 56% aa sequence identity with mouse IFN‑ gamma  R2.  IFN‑ gamma  R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma  R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma  R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma  R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma . (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma . Following expansion, IFN‑ gamma  R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma  signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma  R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8).

  1. Hemmi, S. et al. (1994) Cell 76:803.
  2. Krause, C.D. et al. (2006) Cell Res. 16:55.
  3. Haring, J. S. et al. (2005) J. Immunol. 174:6791.
  4. Tau, G.Z. et al. (2000) J. Exp. Med. 192:977.
  5. Foulds, K.E. et al. (2008) J. Immunol. 180:842.
  6. Rosenzweig, S.D. et al. (2004) J. Immunol. 173:4000.
  7. Filipe-Santos. O. et al. (2006) Semin. Immunol. 18:347.
  8. Zhang, S-Y. et al. (2008) Immunol. Rev. 226:29.

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