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Recombinant Human GLP-1R His-tag Protein, CF

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When GLP-1 peptide (5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) binds with an ED50 of 5.00-30.0 ng/mL.
When GLP-1 peptide (5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) binds with an ED50 of 5.00-30.0 ng/mL.
2 μg/lane of Recombinant Human GLP-1R His-tag Protein (Catalog # 10956-GL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands ...read more

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human GLP-1R His-tag Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. When GLP-1 peptide (Tocris, Catalog # 5374) is immobilized at 0.250 µg/mL (100 µL/well), Recombinant Human GLP-1R His-tag binds with an ED50 of 5.00-30.0 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human GLP-1R protein
Ala2-Glu139, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala2 & Arg24
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa & 15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
40-50 kDa, under reducing conditions.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human GLP-1R His-tag Protein, CF

  • GLP-1 receptor
  • GLP1R
  • GLP-1R
  • GLP-1-R
  • glucagon-like peptide 1 receptor
  • MGC138331

Background

Glucagon-like Peptide 1 Receptor (GLP1R) is a member of the Class B or secretin receptor-like family of G protein-coupled receptors (GPCRs) (1-3). Activation of GLP1R signaling regulates secretion of insulin from pancreatic beta -cells in a glucose-dependent manner (4). Human GLP1R consists of an N-terminal, extracellular domain (ECD) containing 6 conserved cysteine residues and a transmembrane domain with seven membrane-spanning alpha-helices, characteristic of GPCRs (1, 3, 5). The mature ECD of human GLP1R shares 87% and 86% amino acid identity with mouse and rat GLP1R, respectively. GLP1R is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach and it is a receptor for Glucagon-like Peptide, a regulator of insulin secretion by pancreatic beta cells (2, 5, 6). GLP1R agonists, including GLP1, Boc5, and Cpd1, have been targeted for research and used as type 2 diabetes treatments (6, 7). GLP1R is also expressed in the brain where it is involved in the control of appetite (7).
  1. Ma, H. et al. (2020) Cell Research. 30:1140.
  2. Song, G. et al. (2017) Nature. 546:312.
  3. Hollenstein, K. et al. (2013) Trends Pharmacol Sci. 35:12.
  4. Drucker, D.J. et al. (1987) PNAS. 84(10):3434.
  5. Donnelly, D. (2012) Br. J. Pharmacol. 166:27.
  6. Simo, R. and Hernandez, C. (2017) Diabetes. 66:1453.
  7. Holst, J.J. (2004) Expert Opinion on Emerging Drugs. 9:155.

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Recombinant Human
GLP-1R His-tag Protei ...
10956-GL
Recombinant Human GLP-1R His-tag Protein, CF
Species: Hu
Applications: Bioactivity

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