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Recombinant Human Galectin-3 Protein, CF

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Recombinant Human Galectin-3 (Catalog # 1154-GA/CF) agglutinates human red blood cells. The ED50 for this effectis 3-10 µg/mL.
Recombinant Human Galectin-3 (Catalog # 1154-GA/CF) agglutinates human red blood cells. The ED50 for this effectis 3-10 µg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
1154-GA-050
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Recombinant Human Galectin-3 Protein, CF Summary

Additional Information
A New rh Galectin-3 is Now Available! Improved activity and purity; Mammalian cell expressed!
Details of Functionality
Measured by its ability to agglutinate human red blood cells. Hadari, Y.R. et al. (2000) J. Cell Sci. 113:2385. The ED50 for this effect is 3‑10 µg/mL.
Source
E. coli-derived human Galectin-3 protein
Ala2-Ile250
Accession #
N-terminal Sequence
Ala2
Protein/Peptide Type
Recombinant Proteins
Gene
LGALS3
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
1154-GA/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS and Betamercaptoethanol.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Galectin-3 Protein, CF

  • AGE-R3
  • Carbohydrate-binding protein 35
  • CBP 35,35 kDa lectin
  • CBP35
  • GAL3
  • Gal-3
  • galactin-3
  • Galactose-specific lectin 3
  • Galactoside-binding protein
  • GALBPCBP35
  • Galectin3
  • Galectin-3
  • GALIG
  • IgE-binding protein
  • L29
  • L31
  • L-31
  • Laminin-binding protein
  • Lectin L-29
  • lectin, galactoside-binding, soluble, 3
  • LGALS2
  • LGALS3
  • Mac-2 antigen
  • Mac-2
  • MAC2GAL3

Background

Human Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a nonlectin domain (1, 2). Mature human Galectin-3 shares 78% and 79% amino acid (aa) sequence identity with mouse and rat Galectin-3, respectively. Human Galectin-3 is a 26 kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosacchraides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

 

  1. Robertson, M.W. et al. (1990) Biochemistry 29:8093.
  2. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679.
  3. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181.
  4. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172.
  5. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305.
  6. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616.
  7. Sano, H. et al. (2003) J. Clin. Invest. 112:389.
  8. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841.
  9. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264.
  10. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115.
  11. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177.
  12. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160.
  13. Kohatsu, L. et al. (2006) J. Immunol. 177:4718.
  14. Nieminen, J. et al. (2008) J. Immunol. 180:2466.
  15. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288.
  16. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123.
  17. Sherwi, N. et al. (2012) Future Cardiol. 8:885.
  18. Shin, T. (2013) Acta Histochem. 115:407.

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Publications for Galectin-3 (1154-GA/CF)(9)

We have publications tested in 2 confirmed species: Human, Bacteria.

We have publications tested in 4 applications: Bioassay, ELISA Standard, Enzyme Assay, Surface Plasmon Resonance.


Filter By Application
Bioassay
(7)
ELISA Standard
(1)
Enzyme Assay
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(8)
Bacteria
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using 1154-GA/CF Applications Species
N Freitag, I Tirado-Gon, G Barrientos, KL Powell, P Boehm-Stur, SP Koch, K Hecher, AC Staff, PC Arck, A Diemert, SM Blois Galectin-3 deficiency in pregnancy increases the risk of fetal growth restriction (FGR) via placental insufficiency Cell Death Dis, 2020-07-23;11(7):560. 2020-07-23 [PMID: 32703931] (ELISA Standard, Human) ELISA Standard Human
J Terävä, L Tiainen, U Lamminmäki, PL Kellokumpu, K Pettersson, K Gidwani Lectin nanoparticle assays for detecting breast cancer-associated glycovariants of cancer antigen 15-3 (CA15-3) in human plasma PLoS ONE, 2019-07-25;14(7):e0219480. 2019-07-25 [PMID: 31344060] (Bioassay, Human) Bioassay Human
J Ibarrola, R Sádaba, A Garcia-Peñ, V Arrieta, E Martinez-M, V Alvarez, A Fernández-, A Gainza, E Santamaría, J Fernández-, V Cachofeiro, R Fay, P Rossignol, N López-Andr A role for fumarate hydratase in mediating oxidative effects of galectin-3 in human cardiac fibroblasts Int. J. Cardiol., 2018-05-01;258(0):217-223. 2018-05-01 [PMID: 29544935] (Bioassay, Human) Bioassay Human
L Seguin, MF Camargo, HI Wettersten, S Kato, JS Desgrosell, T von Schals, KC Elliott, E Cosset, J Lesperance, SM Weis, DA Cheresh Galectin-3, a druggable vulnerability for KRAS-addicted cancers Cancer Discov, 2017-09-11;0(0):. 2017-09-11 [PMID: 28893801] (Bioassay, Human) Bioassay Human
J Zhao, B Shiratori, H Chagan-Yas, M Matsumoto, T Niki, M Tanaka, Y Takahashi, O Usami, Y Ashino, T Hattori Secretion of IFN-? Associated with Galectin-9 Production by Pleural Fluid Cells from a Patient with Extrapulmonary Tuberculosis Int J Mol Sci, 2017-06-28;18(7):. 2017-06-28 [PMID: 28657598] (Bioassay, Human) Bioassay Human
Mattsson J, Ravela S, Hekim C, Jonsson M, Malm J, Narvanen A, Stenman U, Koistinen H Proteolytic activity of prostate-specific antigen (PSA) towards protein substrates and effect of peptides stimulating PSA activity. PLoS ONE, 2014-09-19;9(9):e107819. 2014-09-19 [PMID: 25237904] (Bioassay, Enzyme Assay, Human) Bioassay, Enzyme Assay Human
Mackinnon , Alison C, Liu , Xiaojun, Hadoke , Patrick, Miller , Mark R, Newby , David E, Sethi , Tariq Inhibition of galectin-3 reduces atherosclerosis in apolipoprotein E-deficient mice. Glycobiology, 2013-02-19;23(6):654-63. 2013-02-19 [PMID: 23426722] (Bioassay, Human) Bioassay Human
Debierre-Grockiego F, Niehus S, Coddeville B, Elass E, Poirier F, Weingart R, Schmidt RR, Mazurier J, Guerardel Y, Schwarz RT Binding of Toxoplasma gondii glycosylphosphatidylinositols to galectin-3 is required for their recognition by macrophages. J. Biol. Chem., 2010-08-20;285(43):32744-50. 2010-08-20 [PMID: 20729207] (Surface Plasmon Resonance, Bacteria) Surface Plasmon Resonance Bacteria
Papaspyridonos M, McNeill E, de Bono JP, Smith A, Burnand KG, Channon KM, Greaves DR Galectin-3 is an amplifier of inflammation in atherosclerotic plaque progression through macrophage activation and monocyte chemoattraction. Arterioscler. Thromb. Vasc. Biol., 2007-12-20;28(3):433-40. 2007-12-20 [PMID: 18096829] (Bioassay, Human) Bioassay Human

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Bioinformatics

Gene Symbol LGALS3
Uniprot