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Recombinant Human Fibulin 2 Protein, CF

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Recombinant Human Fibulin 2 (Catalog # 9559‑FB) supportsHFL1 human fetal lung fibroblast cell adhesion. The ED50 for this effect is0.15‑0.9 μg/mL
Recombinant Human Fibulin 2 (Catalog # 9559‑FB) supportsHFL1 human fetal lung fibroblast cell adhesion. The ED50 for this effect is0.15‑0.9 μg/mL

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Fibulin 2 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells. The ED50 for this effect is 0.15-0.9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human Fibulin 2 protein
Ala28-Leu1184 (Thr854Ala), with an N-terminal HA tag
Accession #
N-terminal Sequence
Tyr (of HA tag)
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
125 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
150-175 kDa, reducing conditions
Publications
Read Publication using
9559-FB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Fibulin 2 Protein, CF

  • FBLN2
  • FIBL-2
  • Fibulin 2
  • Fibulin-2

Background

Fibulin 2 is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. Fibulin-2 consists of 1184 amino acids (aa) with a predicted molecular weight of ~125 kDa. The overall structure is common to fibulins: 3 Anaphylatoxin (AT)-like domains, 11 Epidermal Growth Factor (EGF)‑like domains, and a Fibulin-type carboxy-terminal (FC) domain (1-4). Fibulin 2 is considered a Class I fibulin because the 400-residue N-terminus is divided into the Na and Nb sections, with the Na section containing 150-residues and 12 cysteines while the remaining Nb section is cysteine-free (2, 3). The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer (5). Fibulin 2 is highly conserved across species, with the human protein sharing 82% amino acid identity compared to both mouse and rat. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined (6). Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin (1, 2, 4, 7). It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors (1, 3, 4). There is also evidence that suggests that Fibulin 2 may play an indirect role in the neurogenesis of adult neural stem cells via interaction with integrins and TGF-beta 1 (8).
  1. Alcendor et al. (2011) Am. J. Pathol. 179:1443.
  2. Timpl et al. (2003) Nat. Rev. Mol. Cell Biol. 4:479.
  3. Kanan et al. (2014) J. Biol. Chem. 289:13419.
  4. Argraves et al. (2003) EMBO reports 4:1127.
  5. Pan et al. (1993) J. Cell Biol. 123:1269.
  6. Fontanil et al. (2014) Oncotarget 5:1253.
  7. El-Hallous et al. (2007) J. Biol. Chem. 282:8935.
  8. Radice et al. (2015) Mol. Cell. Neurosci. 67:75.

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Publications for Fibulin 2 (9559-FB)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: Bioassay.


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